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Team:The University of Alberta/24 June 2008
From 2008.igem.org
(Difference between revisions)
(New page: ==Today== *Got the sequencing results from the sequencing done Friday/Saturday/Monday. Another case of LLC (Looks Like Crap). Winnie is redoing the whole shebang (colony PCR -> gel purific...) |
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*Took the sequencing of the TetR brick up to the MBSU for sequencing. Will have the results tomorrow. | *Took the sequencing of the TetR brick up to the MBSU for sequencing. Will have the results tomorrow. | ||
*The O/Ns for protein expression/Ni-NTA column purification: not all of them grew. The three in J61003 grew, but only two of the I0500 cultures grew - 25 and 35. The plates many of the I0500 cells were taken from were plated beore we found out that we were using too little Kanamycin so it is possible that the colonies were not Kan resistant (and consequently, not the correct parts) to begin with. The ones that did grow were induced to switch to high-copy plasmid mode using IPTG and induced protein expression by adding arabinose. | *The O/Ns for protein expression/Ni-NTA column purification: not all of them grew. The three in J61003 grew, but only two of the I0500 cultures grew - 25 and 35. The plates many of the I0500 cells were taken from were plated beore we found out that we were using too little Kanamycin so it is possible that the colonies were not Kan resistant (and consequently, not the correct parts) to begin with. The ones that did grow were induced to switch to high-copy plasmid mode using IPTG and induced protein expression by adding arabinose. | ||
| - | *Jason is transforming J45700 (wintergreen brick) from the iGEM Parts Binder. | + | *Jason is transforming J45700 (wintergreen brick)and R0040 (ptetR) from the iGEM Parts Binder. |
*Jason finished the primer design he was doing yesterday | *Jason finished the primer design he was doing yesterday | ||
Revision as of 17:10, 24 June 2008
Today
- Got the sequencing results from the sequencing done Friday/Saturday/Monday. Another case of LLC (Looks Like Crap). Winnie is redoing the whole shebang (colony PCR -> gel purification -> sequencing)
- Took the sequencing of the TetR brick up to the MBSU for sequencing. Will have the results tomorrow.
- The O/Ns for protein expression/Ni-NTA column purification: not all of them grew. The three in J61003 grew, but only two of the I0500 cultures grew - 25 and 35. The plates many of the I0500 cells were taken from were plated beore we found out that we were using too little Kanamycin so it is possible that the colonies were not Kan resistant (and consequently, not the correct parts) to begin with. The ones that did grow were induced to switch to high-copy plasmid mode using IPTG and induced protein expression by adding arabinose.
- Jason is transforming J45700 (wintergreen brick)and R0040 (ptetR) from the iGEM Parts Binder.
- Jason finished the primer design he was doing yesterday
