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Template:Team:UC Berkeley/Notebook/SC construction
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| - | + | pBca1256-K112000 | |
| + | |||
| + | PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112000 | ||
| + | ---------------------------------------- | ||
| + | Forward Biobricking of {T4 Holin!} | ||
| + | ccagtGAATTCatgAGATCTatggcagcacctagaatatc | ||
| + | Reverse Biobricking of {T4 Holin!} | ||
| + | cgttaGGATCCttatttagcccttcctaatattctggccgcc | ||
Revision as of 18:40, 24 June 2008
pBca1256-K112000
PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112000
Forward Biobricking of {T4 Holin!} ccagtGAATTCatgAGATCTatggcagcacctagaatatc Reverse Biobricking of {T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc
