Team:BCCS-Bristol/Parts

From 2008.igem.org

(Difference between revisions)
m (Submitted Bio-Bricks)
 
(12 intermediate revisions not shown)
Line 1: Line 1:
-
<html><link rel="stylesheet" href="http://www.chofski.co.uk/iGEM/bccs-igem.css" type="text/css"></html>
+
<html><link rel="stylesheet" href="http://homepage.mac.com/tgorochowski/iGEM/bccs-igem.css" type="text/css"></html>
<div class="bccsNavBar">
<div class="bccsNavBar">
{|  align="center"
{|  align="center"
Line 7: Line 7:
!align="center"|[[Team:BCCS-Bristol/Parts|Submitted Parts]]
!align="center"|[[Team:BCCS-Bristol/Parts|Submitted Parts]]
!align="center"|[[Team:BCCS-Bristol/Modeling|Modelling]]
!align="center"|[[Team:BCCS-Bristol/Modeling|Modelling]]
-
!align="center"|[[Team:BCCS-Bristol/Notebook|Calendar & Notebook]]
+
!align="center"|[[Team:BCCS-Bristol/Notebook|Wet Lab]]
 +
!align="center"|[[Team:BCCS-Bristol/Calendar|Calendar]]
!align="center"|[[Team:BCCS-Bristol/Misc|Miscellaneous]]
!align="center"|[[Team:BCCS-Bristol/Misc|Miscellaneous]]
|}
|}
Line 19: Line 20:
<div class="bccsMain">
<div class="bccsMain">
-
No parts submitted so far.
+
== Submitted Bio-Bricks ==
 +
 
 +
[http://partsregistry.org/Part:BBa_K135000 BBa_K135000]
 +
 
 +
This BioBrick contains pCpxR, a promoter which is responsive to phosphorylated CpxR (CpxR-P). Upon adhesion to hydrophobic surfaces, the CpxRA two-component system is activated. The inner membrane histidine kinase CpxA phosphorylates CpxR which then becomes active and regulates a number of target genes. This promoter is therefore sensitive to adhesion to hydrophobic surfaces.
 +
 
 +
 
 +
[http://partsregistry.org/Part:BBa_K135001 BBa_K135001]
 +
 
 +
In this part, the CpxR-P responsive promoter pCpxR is fused to the BioBrick [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] which encodes GFP. Thus, GFP is expressed after binding to hydrophobic surfaces (see also [http://partsregistry.org/Part:BBa_K135000 BBa_K135000] for further explanations). 
 +
 
 +
 
 +
 
 +
 
<br>
<br>
</div>
</div>

Latest revision as of 22:36, 28 October 2008

BCCS-Parts-Header 01.jpg BCCS-Parts-Header 02.jpg BCCS-Parts-Header 03.jpg

Submitted Bio-Bricks

[http://partsregistry.org/Part:BBa_K135000 BBa_K135000]

This BioBrick contains pCpxR, a promoter which is responsive to phosphorylated CpxR (CpxR-P). Upon adhesion to hydrophobic surfaces, the CpxRA two-component system is activated. The inner membrane histidine kinase CpxA phosphorylates CpxR which then becomes active and regulates a number of target genes. This promoter is therefore sensitive to adhesion to hydrophobic surfaces.


[http://partsregistry.org/Part:BBa_K135001 BBa_K135001]

In this part, the CpxR-P responsive promoter pCpxR is fused to the BioBrick [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] which encodes GFP. Thus, GFP is expressed after binding to hydrophobic surfaces (see also [http://partsregistry.org/Part:BBa_K135000 BBa_K135000] for further explanations).