"
Team:BCCS-Bristol/Parts
From 2008.igem.org
Tgorochowski (Talk | contribs) |
m (→Submitted Bio-Bricks) |
||
| (4 intermediate revisions not shown) | |||
| Line 22: | Line 22: | ||
== Submitted Bio-Bricks == | == Submitted Bio-Bricks == | ||
| - | + | [http://partsregistry.org/Part:BBa_K135000 BBa_K135000] | |
| + | |||
| + | This BioBrick contains pCpxR, a promoter which is responsive to phosphorylated CpxR (CpxR-P). Upon adhesion to hydrophobic surfaces, the CpxRA two-component system is activated. The inner membrane histidine kinase CpxA phosphorylates CpxR which then becomes active and regulates a number of target genes. This promoter is therefore sensitive to adhesion to hydrophobic surfaces. | ||
| + | |||
| + | |||
| + | [http://partsregistry.org/Part:BBa_K135001 BBa_K135001] | ||
| + | |||
| + | In this part, the CpxR-P responsive promoter pCpxR is fused to the BioBrick [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] which encodes GFP. Thus, GFP is expressed after binding to hydrophobic surfaces (see also [http://partsregistry.org/Part:BBa_K135000 BBa_K135000] for further explanations). | ||
| + | |||
| + | |||
| + | |||
| + | |||
<br> | <br> | ||
</div> | </div> | ||
Latest revision as of 22:36, 28 October 2008
| Home | The Team | The Project | Submitted Parts | Modelling | Wet Lab | Calendar | Miscellaneous |
|---|
  
|
Submitted Bio-Bricks
[http://partsregistry.org/Part:BBa_K135000 BBa_K135000]
This BioBrick contains pCpxR, a promoter which is responsive to phosphorylated CpxR (CpxR-P). Upon adhesion to hydrophobic surfaces, the CpxRA two-component system is activated. The inner membrane histidine kinase CpxA phosphorylates CpxR which then becomes active and regulates a number of target genes. This promoter is therefore sensitive to adhesion to hydrophobic surfaces.
[http://partsregistry.org/Part:BBa_K135001 BBa_K135001]
In this part, the CpxR-P responsive promoter pCpxR is fused to the BioBrick [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] which encodes GFP. Thus, GFP is expressed after binding to hydrophobic surfaces (see also [http://partsregistry.org/Part:BBa_K135000 BBa_K135000] for further explanations).
