Team:University of Ottawa/25 June 2008

From 2008.igem.org

(Difference between revisions)
(New page: __TOC__ ==Today in the Lab== '''Matt''' :'''PCR Amplification''' ::<li> F60, F61 primers used for PTP2 cassette. I also labelled all the primers we received that we will be using for the n...)
 
(5 intermediate revisions not shown)
Line 1: Line 1:
 +
{|######Navigational Bar#######
 +
|}
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
<title>Untitled Document</title>
 +
<script src="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBar.js" type="text/javascript"></script>
 +
<link href="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarHorizontal.css" rel="stylesheet" type="text/css" />
 +
</head>
 +
 +
<body>
 +
 +
<ul id="MenuBar1" class="MenuBarHorizontal">
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa">Home</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Welcome_to_the_uOttawa_Team_Wiki.21">Welcome</a></li>
 +
        </ul>
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa/Team">The Team</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Who_we_are">Who We Are</a>
 +
            <ul>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Advisors">Advisors</a></li>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Undergrads">Undergrads</a></li>
 +
            </ul>
 +
        </li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#What_we_did">What We've Done</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Where_we.27re_from">Where We're From</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Contact_Us">Contact Us</a></li>
 +
       
 +
      </ul>
 +
  </li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Project">The Project</a>
 +
      <ul>
 +
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#The_Template">Template</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#The_Design">Design</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#References">References</a></li>
 +
      </ul>
 +
  </li>
 +
  <li><a href="http://partsregistry.org/Part:BBa_K149001:Design">BioBricks</a></li>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a>
 +
    <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li>
 +
    </ul>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li>   
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li>       
 +
      </ul>
 +
  </li>
 +
 +
</ul>
 +
<p>&nbsp;</p>
 +
<p>&nbsp;</p>
 +
<script type="text/javascript">
 +
<!--
 +
var MenuBar1 = new Spry.Widget.MenuBar("MenuBar1", {imgDown:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarDownHover.gif", imgRight:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarRightHover.gif"});
 +
//-->
 +
</script>
 +
</body>
 +
</html>
 +
__TOC__
__TOC__
==Today in the Lab==
==Today in the Lab==
Line 8: Line 76:
:'''Glycerol Stock'''
:'''Glycerol Stock'''
::<li>Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.
::<li>Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.
 +
'''Dan'''
 +
:'''Ran gel of pSSA42, S1, D12, T123 plasmids'''
 +
::<li> This time the marker was added and expected results were obtained. SB, DA, TB, and 42B colonies were chosen for glycerol stock.
 +
:'''Cleaned out DNA box'''
 +
::<li> Cleaned out all DNA that will not be used from our box
 +
::<li> Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
 +
:::{| class="wikitable"
 +
|-
 +
| '''Plasmid''' || '''Strain''' || '''New Name'''
 +
|-
 +
| 587 || 7B || 7
 +
|-
 +
| 598 || 8B || 8
 +
|-
 +
| 600 || 0A || 0
 +
|-
 +
| 601 || 1C || 1
 +
|-
 +
| S1 || SB || S
 +
|-
 +
| D12 || DA || D
 +
|-
 +
| T123 || TB || T
 +
|-
 +
| pSSA42 || 42B || 42
 +
|}
 +
:'''PCR amplification of S, D, T, 7 and 8'''
 +
::<li> S, D, and T plasmids were PCR amplified overnight using primers F69 and F70
 +
::<li> PCR program for 0&1 was: PHUSION
 +
::<li> PCR program for S&D&T was: PHN1
 +
::<li> The 0 and 1 plasmids were amplified according to the following chart: (Primer F66 is :Int TetR Repl F (*PstI) (B). primer F67 is Int GFP Repl F(*SphI) (A), and primer F68 is:Int SSRE R(*ClaI )
 +
:::{| class="wikitable"
 +
|-
 +
| '''Sample''' || '''F66''' || '''F67''' || '''F68'''
 +
|-
 +
| 0A ||    || Y || Y
 +
|-
 +
| 1A ||    || Y || Y
 +
|-
 +
| 0B || Y ||    || Y
 +
|-
 +
| 1B || Y ||    || Y
 +
|}

Latest revision as of 23:38, 28 October 2008

Untitled Document

 

 

Contents

Today in the Lab

Matt

PCR Amplification
  • F60, F61 primers used for PTP2 cassette. I also labelled all the primers we received that we will be using for the next few months.
  • I decided to use the Kaern Lab protocol for PCR Amplification.
  • The PTP2 PCR product was then run on a gel - gel results were as expected with 2275 size band.
    • Glycerol Stock
  • Glycerol stock of plasmids D12, S1, T123, pSS042 with LB + glycerol was performed.

    • Dan

    Ran gel of pSSA42, S1, D12, T123 plasmids
  • This time the marker was added and expected results were obtained. SB, DA, TB, and 42B colonies were chosen for glycerol stock.
    • Cleaned out DNA box
  • Cleaned out all DNA that will not be used from our box
  • Our names are getting complicated so I am renaming our DNA using the following legend (all other DNA was discarded)
    • Plasmid Strain New Name
    587 7B 7
    598 8B 8
    600 0A 0
    601 1C 1
    S1 SB S
    D12 DA D
    T123 TB T
    pSSA42 42B 42
    PCR amplification of S, D, T, 7 and 8
  • S, D, and T plasmids were PCR amplified overnight using primers F69 and F70
  • PCR program for 0&1 was: PHUSION
  • PCR program for S&D&T was: PHN1
  • The 0 and 1 plasmids were amplified according to the following chart: (Primer F66 is :Int TetR Repl F (*PstI) (B). primer F67 is Int GFP Repl F(*SphI) (A), and primer F68 is:Int SSRE R(*ClaI )
    • Sample F66 F67 F68
    0A Y Y
    1A Y Y
    0B Y Y
    1B Y Y
    Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/25_June_2008"