Team:Hawaii/Protocols/Solid growth media

From 2008.igem.org

(Difference between revisions)
(New page: ==LB agar== ===Materials=== 25 g LB broth mixture (Difco) 1 L ddH<sub>2</sub>O === Procedure=== # Mix dry ingredients together with 1 L ddH<sub>2</sub>O using a magnetic stir bar. # Autoc...)
(→Materials)
Line 1: Line 1:
==LB agar==
==LB agar==
===Materials===
===Materials===
-
25 g LB broth mixture (Difco)
+
* 25 g LB broth mixture (Difco)
-
1 L ddH<sub>2</sub>O
+
* 1 L ddH<sub>2</sub>O
=== Procedure===
=== Procedure===

Revision as of 22:58, 24 June 2008

Contents

LB agar

Materials

  • 25 g LB broth mixture (Difco)
  • 1 L ddH2O

Procedure

  1. Mix dry ingredients together with 1 L ddH2O using a magnetic stir bar.
  2. Autoclave to sterilize. Let cool to 55C.
  3. Pour 28 ml LB solution for thin plates; 50 ml for thick plates.

LB + amp100

Materials

1 L LB agar 1 ml 1000X ampicillin stock solution

  1. Make LB agar as directed.
  2. When mixture is cooled to 55C, add 1 ml 1000X ampicilllin stock solution.
  3. Plate as usual.

LB+kan20+amp50

Materials

1 L LB agar 0.5 mL 1000X ampicillin stock solution

Retrieved from "http://2008.igem.org/Team:Hawaii/Protocols/Solid_growth_media"