Wiki/Team:Warsaw/igem notebook.htm

From 2008.igem.org

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<tdalign=center><h2>Table of contents</h2></td><td>&nbsp;</td></tr>
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<td width=70% valign=top style="border-left-style: grooved">
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<h3><a onClick=switchMenu('one-armed-vect')> Preparation of one-armed bandit vectors</a></h3>
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<div name="menu_item" style="display: none" id="one-armed-vect" >
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<ol>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=6_May_2008&arg1=7_May_2008&arg2=9_May_2008&arg3=12_May_2008&arg4=13_May_2008&arg5=14_May_2008&name=Preparation%20of%20pMPMT5%20AID%20construct>pMPMT5+AID</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=3_June_2008&arg1=4_June_2008&arg2=5_June_2008&name=Preparation%20of%20pMPMT5%20T7%20construct>pMPMT5-T7</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=16&arg0=5_May_2008&arg1=6_May_2008&arg2=8_May_2008&arg3=13_May_2008&arg4=14_May_2008&arg5=15_May_2008&arg6=16_May_2008&arg7=19_May_2008&arg8=20_May_2008&arg9=21_May_2008&arg10=22_May_2008&arg11=23_May_2008&arg12=24_May_2008&arg13=26_May_2008&arg14=27_May_2008&arg15=28_May_2008&name=Preparation%20of%20pMPMT5-AID%20T7%20and%20pMPMT5%20AID-T7>pMPMT5+AID+T7 and pMPM+AID-T7</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=2_June_2008&arg1=3_June_2008&arg2=4_June_2008&arg3=5_June_2008&arg4=6_June_2008&arg5=9_June_2008&name=Preparation%20of%20pMPMT5-AID%20AIDT7%20construct>pMPMT5+AID+AID-T7</a>
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</li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=25_June_2008&arg1=26_June_2008&arg2=27_June_2008&arg3=30_June_2008&arg4=1_July_2008&arg5=2_July_2008&arg6=3_July_2008&arg7=4_July_2008&name=Change%20of%20the%20reporter%20from%20pZC320%20with%20B-galactosidase%20to%20GFP%20or%20RFP> Change of the reporter from pZC320 with B-galactosidase to GFP or RFP</a></li>
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</ol>
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</div>
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<h3><a OnClick=switchMenu('one-armed-tests')> One-armed bandit tests</a></h3>
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<div name="menu_item" style="display: none" id="one-armed-tests">
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<ol>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=19_May_2008&arg1=20_May_2008&arg2=21_May_2008&name=Rifampicin%20test%20%231>Rifampicin Test #1 </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=22_May_2008&arg1=23_May_2008&arg2=24_May_2008&name=Rifampicin%20test%20%232>Rifampicin Test #2 </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=28_May_2008&arg1=29_May_2008&arg2=2_June_2008&name=Rifampicin%20test%20%233>Rifampicin Test #3 </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=2_June_2008&arg1=5_June_2008&arg2=6_June_2008&arg3=11_June_2008&arg4=12_June_2008&arg5=13_June_2008&arg6=16_June_2008&name=Blue/white%20and%20rifampicin%20test%20%231>Blue/white and Rifampicin test#1 </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=17_June_2008&arg1=18_June_2008&arg2=19_June_2008&arg3=23_June_2008&name=Blue/white%20and%20rifampicin%20test%20%232>Blue/white and Rifampicin test#2 </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=29_August_2008&arg1=30_August_2008&arg2=31_August_2008&arg3=1_September_2008&name=Blue-white%20screening%20and%20rifampicin%20test%20in%20GM2163>Blue-white screening and rifampicin test in GM2163 </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=17_September_2008&arg1=18_September_2008&arg2=19_September_2008&arg3=22_September_2008&arg4=23_September_2008&arg5=24_September_2008&arg6=25_September_2008&arg7=29_September_2008&name=MutD5%20testing>MutD5 testing </a></li>
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</ol>
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</div>
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<h3><a OnClick=switchMenu('hunter-vect')>Preparation of vectors for 'hunter' part of 'hunter and prey'</a></h3>
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<div name="menu_item" id="hunter-vect" style="display: none">
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<ol>
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<li class="nb_toc1"><a OnClick=switchMenu('prot_A','submenu_item')>Preparation of vectors with protein A derivatives fusions</a></li>
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<div name="submenu_item" id="prot_A" style="display: none">
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<ul>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=10_June_2008&name=Design%20of%20primers%20for%20fusion%20of%20protein%20A%2C%20OmpA%20and%20alpha%20and%20omega%20fragments%20of%20beta-lactamase>Design of primers for fusion of protein A, OmpA and alpha and omega fragments of &beta;-lactamase</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=20_June_2008&arg1=23_June_2008&arg2=24_June_2008&arg3=25_June_2008&arg4=26_June_2008&name=Preparation%20of%20alpha-A%20and%20omega-A%20fusions>Preparation of alpha-A and omega-A fusions</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=27_June_2008&arg1=30_June_2008&arg2=1_July_2008&arg3=3_July_2008&name=Cloning%20alpha-A%20and%20omega-A%20fusions%20on%20pKS>Cloning alpha-A and omega-A fusions on pKS </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A conctruct </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_ΔA DNA fragment to pACYC177 OmpA_alpha </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (&Delta;A) </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid>Cloning of protein A DNA to GeneArt plasmid </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=4&amp;arg0=4_August_2008&amp;arg1=5_August_2008&amp;arg2=6_August_2008&amp;arg3=7_August_2008&amp;name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct">Preparing pACYC177 OmpA_omega_&Delta;A construct</a>
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</li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=18_August_2008&amp;arg1=19_August_2008&amp;arg2=20_August_2008&amp;arg3=21_August_2008&amp;arg4=22_August_2008&amp;name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid">Cloning of protein A DNA to GeneArt plasmid</a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=7&amp;arg0=22_September_2008&amp;arg1=23_September_2008&amp;arg2=24_September_2008&amp;arg3=25_September_2008&amp;arg4=26_September_2008&amp;arg5=27_September_2008&amp;arg6=30_September_2008&amp;name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=24_September_2008&amp;arg1=25_September_2008&amp;name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_ΔA construct </a></li>
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</ul>
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</div>
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<li class="nb_toc1"><a OnClick=switchMenu('prot_Z','submenu_item')>Preparation of vectors with protein Z derivatives fusions</a></li>
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<div name="submenu_item" id="prot_Z" style="display: none">
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<ul>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=13_August_2008&arg1=14_August_2008&arg2=18_August_2008&arg3=19_August_2008&arg4=20_August_2008&arg5=21_August_2008&arg6=22_August_2008&arg7=25_August_2008&arg8=26_August_2008&name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega>Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=9&amp;arg0=13_August_2008&amp;arg1=14_August_2008&amp;arg2=18_August_2008&amp;arg3=19_August_2008&amp;arg4=20_August_2008&amp;arg5=21_August_2008&amp;arg6=22_August_2008&amp;arg7=25_August_2008&amp;arg8=26_August_2008&amp;name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li>
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</ul></div>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=11&arg0=13_June_2008&arg1=16_June_2008&arg2=17_June_2008&arg3=18_June_2008&arg4=19_June_2008&arg5=20_June_2008&arg6=23_June_2008&arg7=24_June_2008&arg8=25_June_2008&arg9=26_June_2008&arg10=27_June_2008&name=Preparation%20of%20constructs%3A%20OmpA_alpha%20and%20OmpA_omega>Preparation of constructs: OmpA_alpha and OmpA_omega</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=7_July_2008&arg1=8_July_2008&arg2=9_July_2008&arg3=10_July_2008&arg4=11_July_2008&arg5=12_July_2008&name=Preparation%20of%20constructs%3A%20OmpA_alpha%20and%20OmpA_omega%20%232>Preparation of constructs: OmpA_alpha and OmpA_omega #2 </a></li>
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</ol>
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</div>
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<h3><a OnClick=switchMenu('prey-vect')>Preparation of vectors for 'prey' part of 'hunter and prey</a></h3>
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<div name="menu_item" id="prey-vect" style="display: none">
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<ol>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_June_2008&arg1=1_July_2008&arg2=2_July_2008&arg3=3_July_2008&arg4=4_July_2008&name=Preparation%20of%20constructs%20on%20pET15b%3A%20OmpA_alpha%20and%20OmpA_omega>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=19_August_2008&arg1=22_August_2008&arg2=25_August_2008&arg3=26_August_2008&arg4=27_August_2008&arg5=28_August_2008&arg6=29_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20pET15b%20OmpA-alpha%20plasmid>Cloning of protein A DNA to pET15b OmpA-alpha plasmid </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-alpha%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=7&amp;arg0=19_August_2008&amp;arg1=22_August_2008&amp;arg2=25_August_2008&amp;arg3=26_August_2008&amp;arg4=27_August_2008&amp;arg5=28_August_2008&amp;arg6=29_August_2008&amp;name=Cloning%20of%20protein%20A%20DNA%20to%20pET15b%20OmpA-alpha%20plasmid">Cloning of protein A DNA to pET15b OmpA-alpha plasmid</a></li>
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</ol>
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</div>
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<h3><a OnClick=switchMenu('prey-purif')>Purification of prey proteins</a></h3>
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<div name="menu_item" id="prey-purif" style="display: none">
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<ol>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega>Purification of proteins: Z-alpha and Z-omega </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=8&amp;arg0=29_August_2008&
 +
amp;arg1=1_September_2008&amp;arg2=2_September_2008&amp;arg3=3_September_2008&amp;arg4=4_September_2008&amp;arg5=5_September_2008&amp;arg6=8_September_2008&amp;arg7=9_September_2008&amp;name=Purification%20of%20proteins%3A%20A-alpha">Purification of proteins: A-alpha</a></li>
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</ol>
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</div>
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<h3><a OnClick="switchMenu('hunter-tests')">'Hunter and prey' tests</h3>
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<div name="menu_item" id="hunter-tests" style="display: none">
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<ol>
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<li class="nb_toc1"><a OnClick="switchMenu('expr-tests','submenu_item')">Protein expression tests</li>
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<div name="submenu_item" id="expr-tests" style="display: none">
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<ul>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=4_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20and%20OmpA_A_alpha%20expression>Checking OmpA_omega_ΔA_alpha and OmpA_A_alpha expression </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_ΔA_alpha expression </a></li>
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</ul>
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</div>
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<li class="nb_toc1"><a OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li>
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<div name="submenu_item" id="resist-tests" style="display: none">
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<ul>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=19_August_2008&arg1=20_August_2008&arg2=21_August_2008&arg3=22_August_2008&arg4=25_August_2008&arg5=26_August_2008&arg6=27_August_2008&arg7=9_September_2008&arg8=10_September_2008&arg9=11_September_2008&name=Tests%20for%20ampicillin%20resistance%20given%20by%20protein%20added%20to%20medium>Tests for ampicillin resistance given by protein added to medium </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&name=Checking%20if%20OmpA_omega_%26Delta%3BA_alpha%20gives%20ampicillin%20resistance>Checking if OmpA_omega_ΔA_alpha gives ampicillin resistance </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=10&amp;arg0=19_August_2008&amp;arg1=20_August_2008&amp;arg2=21_August_2008&amp;arg3=22_August_2008&amp;arg4=25_August_2008&amp;arg5=26_August_2008&amp;arg6=27_August_2008&amp;arg7=9_September_2008&amp;arg8=10_September_2008&amp;arg9=11_September_2008&amp;name=Tests%20for%20ampicillin%20resistance%20given%20by%20protein%20added%20to%20medium">Tests for ampicillin resistance given by protein added to medium</a></li>
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</ul>
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</div>
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 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=4&amp;arg0=6_August_2008&amp;arg1=7_August_2008&amp;arg2=8_August_2008&amp;arg3=11_August_2008&amp;name=Checking%20whether%20degradation%20of%20the%20fusions%20with%20OmpA%20is%20caused%20by%20Lon%20and%20OmpT%20proteases%20%28present%20in%20TOP10%29">Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10)</a></li>
 +
 
 +
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=6_August_2008&arg1=7_August_2008&arg2=8_August_2008&arg3=11_August_2008&name=Checking%20whether%20degradation%20of%20the%20fusions%20with%20OmpA%20is%20caused%20by%20Lon%20and%20OmpT%20proteases%20%28present%20in%20TOP10%29>Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10) </a></li>
 +
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=12_August_2008&arg1=13_August_2008&name=Checking%20for%20presence%20of%20protein%20A%20on%20the%20cell%20membrane>Checking for presence of protein A on the cell membrane </a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=6&amp;arg0=15_September_2008&amp;arg1=16_September_2008&amp;arg2=17_September_2008&amp;arg3=18_September_2008&amp;arg4=19_September_2008&amp;arg5=22_September_2008&amp;name=%27Hunter%20and%20prey%27%20system%20tests%3A%20Competition%20tests">'Hunter and prey' system tests: Competition tests</a></li>
 +
 
 +
 
 +
 
 +
</ol>
 +
</div>
 +
 
 +
<h3><a OnClick=switchMenu('bricks')>Preparation of BioBricks</a></h3>
 +
<div name="menu_item" id="bricks" style="display: none">
 +
 
 +
<ol>
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=17_September_2008&amp;arg1=18_September_2008&amp;name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li>
 +
 
 +
<li class="nb_toc1"><a OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</a></li>
 +
<div name="submenu_item" id="bricks-vect" style="display: none">
 +
<ul>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=3_October_2008&amp;arg1=6_October_2008&amp;arg2=7_October_2008&amp;arg3=8_October_2008&amp;arg4=9_October_2008&amp;name=Preparation%20of%20vector%20for%20pT7%20constructs">Preparation of vector for pT7 constructs</a></li>
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=3&amp;arg0=29_September_2008&amp;arg1=30_September_2008&amp;arg2=1_October_2008&amp;name=Preparation%20of%20vectors%20for%20Biobricks">Preparation of vectors for Biobricks</a></li>
 +
 
 +
</ul>
 +
</div>
 +
 
 +
<li class="nb_toc1"><a OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</a></li>
 +
<div name="submenu_item" id="bricks-coding" style="display: none">
 +
<ul>
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=4&amp;arg0=29_September_2008&amp;arg1=30_September_2008&amp;arg2=1_October_2008&amp;arg3=2_October_2008&amp;name=Preparation%20of%20Z%28BBa_K103004%29">Preparation of Z(BBa_K103004)</a></li>
 +
 
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=1_October_2008&amp;arg1=6_October_2008&amp;arg2=7_October_2008&amp;arg3=8_October_2008&amp;arg4=9_October_2008&amp;name=Preparation%20of%20AID%28BBa_K103001%29">Preparation of AID(BBa_K103001)</a></li>
 +
 
 +
 
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=3&amp;arg0=20_October_2008&amp;arg1=21_October_2008&amp;arg2=22_October_2008&amp;name=Preparation%20of%20OmpA_linker_alpha_linker%20%28BBa_K103014%29">Preparation of OmpA_linker_alpha_linker (BBa_K103014)</a></li>
 +
 
 +
 
 +
 
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=7&amp;arg0=18_September_2008&amp;arg1=19_September_2008&amp;arg2=22_September_2008&amp;arg3=23_September_2008&amp;arg4=24_September_2008&amp;arg5=25_September_2008&amp;arg6=26_September_2008&amp;name=Preparation%20of%20%26Delta%3BA%20%28BBa_K103003%29">Preparation of &Delta;A (BBa_K103003)</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=10&amp;arg0=18_September_2008&amp;arg1=2_October_2008&amp;arg2=3_October_2008&amp;arg3=6_October_2008&amp;arg4=7_October_2008&amp;arg5=8_October_2008&amp;arg6=13_October_2008&amp;arg7=14_October_2008&amp;arg8=15_October_2008&amp;arg9=16_October_2008&amp;name=Preparation%20of%20linker_alpha%20%28BBa_K103009%29">Preparation of linker_alpha (BBa_K103009)</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=6&amp;arg0=18_September_2008&amp;arg1=2_October_2008&amp;arg2=3_October_2008&amp;arg3=6_October_2008&amp;arg4=7_October_2008&amp;arg5=8_October_2008&amp;name=Preparation%20of%20linker_omega%20%28BBa_K103013%29">Preparation of linker_omega (BBa_K103013)</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=15&amp;arg0=18_September_2008&amp;arg1=29_September_2008&amp;arg2=30_September_2008&amp;arg3=2_October_2008&amp;arg4=3_October_2008&amp;arg5=6_October_2008&amp;arg6=7_October_2008&amp;arg7=8_October_2008&amp;arg8=13_October_2008&amp;arg9=14_October_2008&amp;arg10=15_October_2008&amp;arg11=16_October_2008&amp;arg12=17_October_2008&amp;arg13=21_October_2008&amp;arg14=22_October_2008&amp;name=Preparation%20of%20OmpA-linker-omega-linker%20%28BBa_K103016%29">Preparation of OmpA-linker-omega-linker (BBa_K103016)</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=18_September_2008&amp;arg1=29_September_2008&amp;arg2=30_September_2008&amp;arg3=2_October_2008&amp;arg4=3_October_2008&amp;name=Preparation%20of%20OmpA-linker%20%28BBa_K103006%29">Preparation of OmpA-linker (BBa_K103006)</a></li>
 +
</ul>
 +
</div>
 +
 
 +
<li class="nb_toc1"><a OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</a></li>
 +
<div name="submenu_item" id="bricks-generator" style="display: none">
 +
<ul>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=10_October_2008&amp;arg1=13_October_2008&amp;arg2=14_October_2008&amp;arg3=15_October_2008&amp;arg4=16_October_2008&amp;name=Preparation%20of%20OmpA_linker_alpha_linker%20under%20Plac%20%28BBa_K103017%29">Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)</a></li>
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=8&amp;arg0=29_September_2008&amp;arg1=30_September_2008&amp;arg2=1_October_2008&amp;arg3=2_October_2008&amp;arg4=6_October_2008&amp;arg5=7_October_2008&amp;arg6=8_October_2008&amp;arg7=9_October_2008&amp;name=Preparation%20of%20OmpA_linker_omega_linker%20under%20Plac%20%28BBa_K103018%29">Preparation of OmpA_linker_omega_linker under Plac (BBa_K103018)</a></li>
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=9&amp;arg0=25_September_2008&amp;arg1=9_October_2008&amp;arg2=10_October_2008&amp;arg3=13_October_2008&amp;arg4=14_October_2008&amp;arg5=16_October_2008&amp;arg6=17_October_2008&amp;arg7=20_October_2008&amp;arg8=21_October_2008&amp;name=Preparation%20of%20alpha_linker%20under%20PT7%20%28BBa_K103019%29">Preparation of alpha_linker under PT7 (BBa_K103019)</a></li>
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=7&amp;arg0=25_September_2008&amp;arg1=29_September_2008&amp;arg2=30_September_2008&amp;arg3=9_October_2008&amp;arg4=10_October_2008&amp;arg5=13_October_2008&amp;arg6=14_October_2008&amp;name=Preparation%20of%20omega_linker%20under%20PT7%20%28BBa_K103020%29">Preparation of omega_linker under PT7 (BBa_K103020)</a></li>
 +
 
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=12&amp;arg0=7_October_2008&amp;arg1=8_October_2008&amp;arg2=9_October_2008&amp;arg3=10_October_2008&amp;arg4=13_October_2008&amp;arg5=14_October_2008&amp;arg6=15_October_2008&amp;arg7=16_October_2008&amp;arg8=17_October_2008&amp;arg9=20_October_2008&amp;arg10=21_October_2008&amp;arg11=22_October_2008&amp;name=Preparation%20of%20AID%20under%20pBAD/araC%20%28BBa_K103002%29">Preparation of AID under pBAD/araC (BBa_K103002)</a></li>
 +
</ul>
 +
</div>
 +
 
 +
</ol>
 +
 
 +
</div>
 +
 
 +
 
 +
<h3><a OnClick=switchMenu('other')>Other activities</a></h3>
 +
<div name="menu_item" id="other" style="display: none">
 +
<ol>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=6_September_2008&amp;arg1=14_September_2008&amp;name=Wiki%20scripting">Wiki scripting</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=8_September_2008&amp;name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=11_September_2008&amp;name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=23_October_2008&amp;name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=24_October_2008&amp;name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=10_October_2008&amp;name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li>
 +
</ol>
 +
</div>
 +
 
 +
 
 +
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</td></tr></table>
<h2 align="left"><a href=https://2008.igem.org/Wiki/Team:Warsaw/protocols_list>Protocols</a></h2>
<h2 align="left"><a href=https://2008.igem.org/Wiki/Team:Warsaw/protocols_list>Protocols</a></h2>
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Revision as of 00:46, 29 October 2008

Gallery Bricks Notebook Team Project Home

Table of contents

 

Preparation of one-armed bandit vectors

One-armed bandit tests

Preparation of vectors for 'hunter' part of 'hunter and prey'

Preparation of vectors for 'prey' part of 'hunter and prey

Purification of prey proteins

'Hunter and prey' tests

Preparation of BioBricks

Other activities

May
MTWTFSS
      [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_May_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_May_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_May_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_May_2008 4]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/5_May_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_May_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_May_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_May_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_May_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_May_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_May_2008 11]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/12_May_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_May_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_May_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_May_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_May_2008 18]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/19_May_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_May_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_May_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_May_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_May_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_May_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_May_2008 25]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/26_May_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_May_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_May_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_May_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_May_2008 31]
June
MTWTFSS
            [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_June_2008 1]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/2_June_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_June_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_June_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_June_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_June_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_June_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_June_2008 8]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/9_June_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_June_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_June_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_June_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_June_2008 15]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_June_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_June_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_June_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_June_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_June_2008 22]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_June_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_June_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_June_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_June_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_June_2008 29]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_July_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_July_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_July_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_July_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_July_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_July_2008 6]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/7_July_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_July_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_July_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_July_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_July_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_July_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_July_2008 13]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/14_July_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_July_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_July_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_July_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_July_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_July_2008 20]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/21_July_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_July_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_July_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_July_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_July_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_July_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_July_2008 27]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/28_July_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_July_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_July_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_August_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_August_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_August_2008 3]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/4_August_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_August_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_August_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_August_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_August_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_August_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_August_2008 10]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/11_August_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_August_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_August_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_August_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_August_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_August_2008 17]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/18_August_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_August_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_August_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_August_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_August_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_August_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_August_2008 24]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/25_August_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_August_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_August_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_August_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_August_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_August_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_August_2008 31]
September
MTWTFSS
[http://2008.igem.org/Team:Warsaw/Calendar-Main/1_September_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_September_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_September_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_September_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_September_2008 5] [http://2008.igem.org/Team:Warsaw/Calendar-Main/6_September_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_September_2008 7]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/8_September_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_September_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_September_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_September_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_September_2008 12] [http://2008.igem.org/Team:Warsaw/Calendar-Main/13_September_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_September_2008 14]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/15_September_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_September_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_September_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_September_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_September_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_September_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_September_2008 21]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/22_September_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_September_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_September_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_September_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_September_2008 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_September_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_September_2008 28]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/29_September_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/Team:Warsaw/Calendar-Main/1_October_2008 1] [http://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008 2] [http://2008.igem.org/Team:Warsaw/Calendar-Main/3_October_2008 3] [http://2008.igem.org/Team:Warsaw/Calendar-Main/4_October_2008 4] [http://2008.igem.org/Team:Warsaw/Calendar-Main/5_October_2008 5]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/6_October_2008 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_October_2008 7] [http://2008.igem.org/Team:Warsaw/Calendar-Main/8_October_2008 8] [http://2008.igem.org/Team:Warsaw/Calendar-Main/9_October_2008 9] [http://2008.igem.org/Team:Warsaw/Calendar-Main/10_October_2008 10] [http://2008.igem.org/Team:Warsaw/Calendar-Main/11_October_2008 11] [http://2008.igem.org/Team:Warsaw/Calendar-Main/12_October_2008 12]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/13_October_2008 13] [http://2008.igem.org/Team:Warsaw/Calendar-Main/14_October_2008 14] [http://2008.igem.org/Team:Warsaw/Calendar-Main/15_October_2008 15] [http://2008.igem.org/Team:Warsaw/Calendar-Main/16_October_2008 16] [http://2008.igem.org/Team:Warsaw/Calendar-Main/17_October_2008 17] [http://2008.igem.org/Team:Warsaw/Calendar-Main/18_October_2008 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_October_2008 19]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/20_October_2008 20] [http://2008.igem.org/Team:Warsaw/Calendar-Main/21_October_2008 21] [http://2008.igem.org/Team:Warsaw/Calendar-Main/22_October_2008 22] [http://2008.igem.org/Team:Warsaw/Calendar-Main/23_October_2008 23] [http://2008.igem.org/Team:Warsaw/Calendar-Main/24_October_2008 24] [http://2008.igem.org/Team:Warsaw/Calendar-Main/25_October_2008 25] [http://2008.igem.org/Team:Warsaw/Calendar-Main/26_October_2008 26]
[http://2008.igem.org/Team:Warsaw/Calendar-Main/27_October_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_October_2008 28] [http://2008.igem.org/Team:Warsaw/Calendar-Main/29_October_2008 29] [http://2008.igem.org/Team:Warsaw/Calendar-Main/30_October_2008 30] [http://2008.igem.org/Team:Warsaw/Calendar-Main/31_October_2008 31]
November
MTWTFSS
          [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/2_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 2]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2008.igem.org/Team:Warsaw/Calendar-Main/7_November_2008 7] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 9]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/13_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/14_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/15_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/16_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 16]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/17_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 18] [http://2008.igem.org/Team:Warsaw/Calendar-Main/19_November_2008 19] [http://2008.igem.org/Team:Warsaw/Calendar-Main/20_November_2008 20] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/23_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 23]
[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/26_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 26] [http://2008.igem.org/Team:Warsaw/Calendar-Main/27_November_2008 27] [http://2008.igem.org/Team:Warsaw/Calendar-Main/28_November_2008 28] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_November_2008&preload=Team:Warsaw/NotebookPreload&action=edit 30]

Protocols

Primers

Vectors

Strains

Rosetta(DE3)pLysS

F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)

TOP10 (Invitrogen)

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-

NR9458 (IBB collection; in notebook called mutD5)

ara thi Δ pro lac mutD5 zaf-13 ::Tn10

GM2163

ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2