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Team:NTU-Singapore/Notebook/25 June 2008
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| + | *1030am: Hung: take out the agar plates incubated from Tuesday night. For details of the plasmids, please refer to [https://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Notebook/24_June_2008 Tuesday notebook] | ||
| + | **GFP reporters with standard, weak, medium and strong promoters all have colonies. | ||
| + | **GFP reporter device BBa_E2040 however shows no colony. | ||
| + | **GFP producer BBa_E4080 shows a few colonies. | ||
| + | **LacI-GFP shows no colony. | ||
| + | **GFP self-ligation shows a few colonies. | ||
| + | **pFe-GFP shows no colony. | ||
| + | |||
| + | *11am: Group meeting to discuss about current obstacles encountered during wetlab. | ||
| + | |||
| + | *12:15pm: Choon Kit: digestion for: | ||
| + | **pFe with SpeI/PstI | ||
| + | **GFP with XbaI/PstI | ||
| + | **pFe with SpeI to check SpeI activity | ||
| + | **Terminator with SpeI to check SpeI activity<br>Incubate for 3 hours. | ||
Revision as of 04:48, 25 June 2008
Wednesday 25 June
- 1030am: Hung: take out the agar plates incubated from Tuesday night. For details of the plasmids, please refer to Tuesday notebook
- GFP reporters with standard, weak, medium and strong promoters all have colonies.
- GFP reporter device BBa_E2040 however shows no colony.
- GFP producer BBa_E4080 shows a few colonies.
- LacI-GFP shows no colony.
- GFP self-ligation shows a few colonies.
- pFe-GFP shows no colony.
- 11am: Group meeting to discuss about current obstacles encountered during wetlab.
- 12:15pm: Choon Kit: digestion for:
- pFe with SpeI/PstI
- GFP with XbaI/PstI
- pFe with SpeI to check SpeI activity
- Terminator with SpeI to check SpeI activity
Incubate for 3 hours.
