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Wisconsin: Lignin Project/26 June 2008
From 2008.igem.org
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(New page: '''Team Fungus:''' <br> Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1 <br> Perform transformation of plasmid into E. coli (BL21) and plated to grow ove...) |
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| + | |'''Team Sorbitol:''' | ||
| + | Found that creating fresh proteinase K solution every time will work better.<br> | ||
| + | Designed and set up a gradiant PCR (using Herculase).<br> | ||
| + | Several successful bands for ''srlD'' were obtained.<br> | ||
| + | The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample.<br> | ||
| + | Also designed sequencing primers to test for ''srlD'' in both pBAD30 and pBAD18.<br> | ||
| + | Found there was once a commercial kit for quantifying sorbitol (Boehringer Manheim), however it was now not available in the market.<br> | ||
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'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1 <br> | Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1 <br> | ||
Perform transformation of plasmid into E. coli (BL21) and plated to grow overnight | Perform transformation of plasmid into E. coli (BL21) and plated to grow overnight | ||
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Latest revision as of 02:50, 29 October 2008
| Team Sorbitol:
Found that creating fresh proteinase K solution every time will work better. Team Fungus: |
