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Team:UCSF/Parts
From 2008.igem.org
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'''AarI acceptor vectors (empty)''' | '''AarI acceptor vectors (empty)''' | ||
| - | ''These pRS3series vectors accept AarI digested parts | + | ''These pRS3series vectors accept AarI digested parts between a promoter/terminator. The promoter is flanked by PspOMI/XhoI sites, and the terminator by Not1/SacI sites, in case you want to change them out.'' |
*AarI AD Acceptor (pRS305, Gal1P, Adh1t) | *AarI AD Acceptor (pRS305, Gal1P, Adh1t) | ||
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*AarI AD Acceptor (pRS315, Cyc1P, Adh1t) | *AarI AD Acceptor (pRS315, Cyc1P, Adh1t) | ||
| - | + | *AarI AD Acceptor (pRS315, Fig1P, Adh1t) | |
| - | * | + | *AarI AD Acceptor (pRS315, 8X LexA Ops-Cyc1P, Adh1t) |
| - | *AarI AD acceptor | + | *AarI AD acceptor (pRS315, Cyc1P, Adh1t-8XLexA Ops) |
| + | *AarI AD Acceptor (pRS315, 8X LexA Ops-Fig1P, Adh1t) | ||
Revision as of 05:30, 29 October 2008
For complete details and sequence, see the Registry: http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=UCSF&Done=1
Project-relevant AarI Parts
digestion of these plasmids with AarI will release a fragment for AarI cloning.
- LexA (1-87 a.a) AB
- Sir2 BD
- GFP AD
- Ssn8 AD
- Sir3 A!D
- Sir4 AD
AarI acceptor vectors (empty) These pRS3series vectors accept AarI digested parts between a promoter/terminator. The promoter is flanked by PspOMI/XhoI sites, and the terminator by Not1/SacI sites, in case you want to change them out.
- AarI AD Acceptor (pRS305, Gal1P, Adh1t)
- AarI AD Acceptor (pRS315, Adh1P, Adh1t)
- AarI AD Acceptor (pRS315, Cyc1P, Adh1t)
- AarI AD Acceptor (pRS315, Fig1P, Adh1t)
- AarI AD Acceptor (pRS315, 8X LexA Ops-Cyc1P, Adh1t)
- AarI AD acceptor (pRS315, Cyc1P, Adh1t-8XLexA Ops)
- AarI AD Acceptor (pRS315, 8X LexA Ops-Fig1P, Adh1t)
AarI Shuttle Vector
So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks.
- AarI--Biobrick Shuttle AB
- AarI--Biobrick Shuttle BD
Bonus AarI Parts
These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.
Esa1 AB
GFP BD
Sir4
Sir2 AB
mCherry AB
mCherry BD
LexA (1-87) BD
Pif3 AB
PhyB AB
Sas2 AB
Sas2 BD
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