Biobrick

cheZ: This part contains the cheZ operon coding region without an rbs nor terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.

cheY*: This is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part contains the coding region only.

pTetR-rbs-cheZ: This part uses BBa_J61003 as a backbone and cheZ as the inserted gene. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The cheZ operon lacks a terminator.

pTetR-rbs-cheY*: This part uses BBa_J61003 as a backbone and cheY* as the inserted gene. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part lacks a terminator.

pTetR-rbs-lacZ alpha: This is a composite part by putting lacZ alpha fragment and J61003 together. This part lacks a terminator.

pLacI/ara-1: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.

pLacIq-rbs-LacI-terminator: This LacI gene contains a mutated pLacIq promoter which enables high level of transcription. This ensures effective repression of pLacI promoter that might be present.

Rbs-mRFP1-terminator: This part is adapted from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit.

pLacI/ara-1-rbs-luxI-luxR-terminator: This is a quorum sensing composition. Induced by IPTG or arabinose, this part can establish a quorum sensing system, with the luxI protein producing AHL and luxR protein as the AHL density sensor.

plambda(R-O12)-rbs-cheZ: This part contains a lambda promoter that is constitutively on and can be repressed by CI protein. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This part lacks a terminator.

Plux-rbs-CI-terminator-plambda-rbs-cheZ: This is a part in which the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances the cheZ gene is constitutively expressed.

plux-rbs-cheZ-terminator: This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.

plux-rbs-CI-terminator: This part encodes a CI protein that is used to repress lambda promoter. The part is controlled by a lux promoter that can be induced by luxR protein under high AHL concentration.

pLacI/ara-1-rbs-luxI-terminator: A luxI operon controlled by a LacI/ara-1 promoter.

plambda(R-O12)-rbs-lacZ: A lacZ gene with a lambda(R-O12) promoter. This part is constitutively expressed but repressed in the presence of CI protein. This part lacks a terminator.

pLacIq-rbs-lacI-terminator-pLacI-rbs-mRFP1-terminator: This part is composited with a lacI operon and an mRFP operon. The LacI operon contains a mutated lacIq promoter that enables high level of transcription. The mRFP operon is spontaneously repressed by the LacI operon, and can be turned on by inducing with IPTG.

cheZ-pLacI-rbs-mRFP1-terminator: the cheZ gene is installed in front of mRFP operon. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This cheZ gene is without a promoter, rbs or terminator.

cheY*-pLacI-rbs-mRFP1-terminator: the cheY* gene is installed in front of mRFP operon. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein.? This cheY* gene is without a promoter, rbs or terminator.

cheZ-pLacIq-rbs-lacI-terminator: the cheZ gene is installed in front of a lacI operon. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This lacI operon contains a mutated LacIq promoter which enables high level of transcription. The cheZ gene is without a promoter, rbs or terminator.

cheY*-pLacIq-rbs-lacI-terminator: the cheY* gene is installed in front of a lacI operon. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This lacI operon contains a mutated LacIq promoter which enables high level of transcription. The cheY* gene is without a promoter, rbs