Team:Heidelberg/Human Practice/Open Day/Microscopy

From 2008.igem.org

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Here you get an impression on what the pubils explored in the microscopy station. [ [[Team:Heidelberg/Human_Practice/Open_Day| ''back to previous page'']] ]
Here you get an impression on what the pubils explored in the microscopy station. [ [[Team:Heidelberg/Human_Practice/Open_Day| ''back to previous page'']] ]
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Cells taken from the oral mucosa are stained with Hoechst and Lysotracker Red in salt-buffer (Krebs-Henseleit). Hoechst is a blue fluorescend dye intercalating into the minor groove of the DNA, thus visualizing the nucleus. Lysotracker Red consists of a red fluorophore coupled to a weak base that accumulates in acidic organelles like lysosomes. A Leica TCS SP5 confocal microscope is used for image aquisition.
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{| align="center"
{| align="center"
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_bac.gif|thumb|350 px| bacteria out of the mouth- group1_1]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_bac.gif|thumb|350 px|Cells of the oral mucosa covered with bacteria (Hoechst stain) ]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Mundschleimhaut.gif|thumb|350 px| bacteria out of the mouth- group3_1]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Mundschleimhaut.gif|thumb|350 px| Cells of the oral mucosa focused to the nucleus (Hoechst stain)]]
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{| align="center"
{| align="center"
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageBakterien.gif|thumb|350 px| bacteria out of the mouth- staining- group3_2]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageBakterien.gif|thumb|350 px| Montage: Cells of the oral mucosa covered with bacteria. Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageMundschleimhaut.gif|thumb|350 px| bacteria out of the mouth- staining- group3_1]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageMundschleimhaut.gif|thumb|350 px| Cells of the oral mucosa focused to the nucleus. Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.]]
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{| align="center"
{| align="center"
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Group1.gif|thumb|350 px| bacteria out of the mouth- group1_2]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Group1.gif|thumb|350 px| Group 1: Hoechst stain of cells from the oral mucosa]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Group2.gif|thumb|350 px| bacteria out of the mouth- group2_1]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Group2.gif|thumb|350 px| Group 2: Hoechst stain of cells from the oral mucosa]]
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{| align="center"
{| align="center"
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageGroup1.gif|thumb|350 px| bacteria out of the mouth- staining- group1_1]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageGroup1.gif|thumb|350 px| Montage group 1: Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageGroup2.gif|thumb|350 px| bacteria out of the mouth- staining- group1_2]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageGroup2.gif|thumb|350 px| Montage group 2: Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.]]
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{| align="center"
{| align="center"
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Group3_1.gif|thumb|350 px| bacteria out of the mouth- group2_2]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Group3_1.gif|thumb|350 px| Group 3: Hoechst stain of cells from the oral mucosa]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Group3_2.gif|thumb|350 px| bacteria out of the mouth- group3_2]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_Group3_2.gif|thumb|350 px| Group 3: Hoechst stain of cells from the oral mucosa]]
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{| align="center"
{| align="center"
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageGroup3_1.gif|thumb|350 px| bacteria out of the mouth- staining- group2_1]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageGroup3_1.gif|thumb|350 px| Montage group 3: Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageGroup3_2.gif|thumb|350 px| bacteria out of the mouth- staining- group2_2]]
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| style="width:120px;" align="right" rowspan="3"| [[image:mchd_MontageGroup3_2.gif|thumb|350 px| Montage group 3: Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.]]
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[ [[Team:Heidelberg/Human_Practice/Open_Day| ''back to previous page'']] ]
[ [[Team:Heidelberg/Human_Practice/Open_Day| ''back to previous page'']] ]

Latest revision as of 08:35, 29 October 2008


Photo Gallery- Microscopy


Here you get an impression on what the pubils explored in the microscopy station. [ back to previous page ]
Cells taken from the oral mucosa are stained with Hoechst and Lysotracker Red in salt-buffer (Krebs-Henseleit). Hoechst is a blue fluorescend dye intercalating into the minor groove of the DNA, thus visualizing the nucleus. Lysotracker Red consists of a red fluorophore coupled to a weak base that accumulates in acidic organelles like lysosomes. A Leica TCS SP5 confocal microscope is used for image aquisition.

Cells of the oral mucosa covered with bacteria (Hoechst stain)
Cells of the oral mucosa focused to the nucleus (Hoechst stain)
Montage: Cells of the oral mucosa covered with bacteria. Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.
Cells of the oral mucosa focused to the nucleus. Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.
Group 1: Hoechst stain of cells from the oral mucosa
Group 2: Hoechst stain of cells from the oral mucosa
Montage group 1: Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.
Montage group 2: Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.
Group 3: Hoechst stain of cells from the oral mucosa
Group 3: Hoechst stain of cells from the oral mucosa
Montage group 3: Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.
Montage group 3: Blue color indicates the nucleus and bacterial DNA, Red color stains acidic compartments and gives a diffuse background in the cytosol.


[ back to previous page ]

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