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Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)
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[[Team:Chiba/protocol/phenotype/timedelay|more details...]] | [[Team:Chiba/protocol/phenotype/timedelay|more details...]] | ||
| - | ==Results== | + | ==Results and Discussion== |
===Reaction temparature:37°C=== | ===Reaction temparature:37°C=== | ||
*センダーの培養液：500μL、レシーバの培養液：500μL | *センダーの培養液：500μL、レシーバの培養液：500μL | ||
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[[Image:Chiba_talks_JW1908_37_RS1_02.gif|thumb|left|Fig. <br>E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.]] | [[Image:Chiba_talks_JW1908_37_RS1_02.gif|thumb|left|Fig. <br>E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.]] | ||
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===Reaction temparature:30°C=== | ===Reaction temparature:30°C=== | ||
Revision as of 12:22, 29 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Reference | Notebook | Acknowledgements |
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design
Sender
- [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI(BBa_K084007)]
- [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI(BBa_K084008)]
- [http://partsregistry.org/Part:BBa_S03623 ptet+rbs+LuxI(LVA)(BBa_S03623)]
Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
Method
- Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 37°C.
- Measured intensity of green fluorescence at regular time intervals.
Results and Discussion
Reaction temparature:37°C
- センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Reaction temparature:30°C
- センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
>Back to Sender experiment and result
