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Team:Paris/Modeling/More f3 Table
From 2008.igem.org
(Difference between revisions)
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|comments | |comments | ||
|- | |- | ||
| - | | | + | |(''fluorescence'') |
| - | + | |value of the observed fluorescence | |
| - | | | + | |au |
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|need for 20 mesures with well choosen values of [aTc]<sub>i</sub> <br> and for 20 mesures with well choosen values of [arab]<sub>i</sub> <br> and 5x5 measures for the relation below? | |need for 20 mesures with well choosen values of [aTc]<sub>i</sub> <br> and for 20 mesures with well choosen values of [arab]<sub>i</sub> <br> and 5x5 measures for the relation below? | ||
|- | |- | ||
| - | | | + | |''conversion'' |
| - | | | + | |conversion ratio between <br> fluorescence and concentration <br> ↓ gives ↓ |
| - | | | + | |nM.au<sup>-1</sup> |
| - | | | + | |(1/79.429) |
| - | | | + | | |
|- | |- | ||
|[GFP] | |[GFP] | ||
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|nM | |nM | ||
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| - | |||
| - | |||
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|- | |- | ||
| - | | | + | |γ<sub>GFP</sub> |
| - | | | + | |dilution-degradation rate <br> of GFP(mut3b) <br> ↓ gives ↓ |
| - | | | + | |min<sup>-1</sup> |
| - | | | + | |0.0198 |
| + | |Time Cell Division : 35 min. | ||
| + | |- | ||
| + | |ƒ3 | ||
| + | |activity of <br> ''pFlhDC'' '''with RBS E0032''' | ||
| + | |nM.min<sup>-1</sup> | ||
| + | | | ||
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|} | |} | ||
Revision as of 12:53, 29 October 2008
| param | signification | unit | value | comments |
| (fluorescence) | value of the observed fluorescence | au | need for 20 mesures with well choosen values of [aTc]i and for 20 mesures with well choosen values of [arab]i and 5x5 measures for the relation below? | |
| conversion | conversion ratio between fluorescence and concentration ↓ gives ↓ | nM.au-1 | (1/79.429) | |
| [GFP] | GFP concentration at steady-state | nM | ||
| γGFP | dilution-degradation rate of GFP(mut3b) ↓ gives ↓ | min-1 | 0.0198 | Time Cell Division : 35 min. |
| ƒ3 | activity of pFlhDC with RBS E0032 | nM.min-1 |
| param | signification corresponding parameters in the equations | unit | value | comments |
| β22 | total transcription rate of FliA><pFlhDC with RBS B0034 β22 | nM.min-1 | ||
| (K6/{coeffliA}) | activation constant of FliA><pFlhDC K6 | nM | ||
| n6 | complexation order of FliA><pFlhDC n6 | no dimension | ||
| β17 | basal activity of pFlhDC with RBS B0034 β17 | nM.min-1 | ||
| (K15/{coefomp}) | activation constant of OmpR><pFlhDC K15 | nM | ||
| n15 | complexation order of OmpR><pFlhDC n15 | no dimension |
