Team:Paris/Modeling/More f2 Table

From 2008.igem.org

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|comments
|comments
|-
|-
-
|ƒ2
+
|(''fluorescence'')
-
|activity of <br> ''pBad'' '''with RBS E0032'''
+
|value of the observed fluorescence
-
|nM.min<sup>-1</sup>
+
|au
|
|
|need for 20 measures with well choosen [arab]<sub>i</sub>
|need for 20 measures with well choosen [arab]<sub>i</sub>
|-
|-
-
|γ<sub>GFP</sub>
+
|''conversion''
-
|dilution-degradation rate <br> of GFP(mut3b)
+
|conversion ratio between <br> fluorescence and concentration <br> &darr; gives &darr;
-
|min<sup>-1</sup>
+
|nM.au<sup>-1</sup>
-
|0.0198
+
|(1/79.429)
-
|Only Dilution <br> Time Cell Disvision : 35 min.
+
|
|-
|-
|[GFP]
|[GFP]
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|nM
|nM
|
|
-
|need for 20 measures
 
-
|-
 
-
|(''fluorescence'')
 
-
|value of the observed fluorescence
 
-
|au
 
|
|
-
|need for 20 measures
 
|-
|-
-
|''conversion''
+
|γ<sub>GFP</sub>
-
|conversion ratio between <br> fluorescence and concentration
+
|dilution-degradation rate <br> of GFP(mut3b) <br> &darr; gives &darr;
-
|nM.au<sup>-1</sup>
+
|min<sup>-1</sup>
-
|(1/79.429)
+
|0.0198
 +
|Only Dilution <br> Time Cell Disvision : 35 min.
 +
|-
 +
|&#131;2
 +
|activity of <br> ''pBad'' '''with RBS E0032'''
 +
|nM.min<sup>-1</sup>
 +
|
|
|
|}
|}
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|-
|-
|β<sub>bad</sub>
|β<sub>bad</sub>
-
|total transcription rate of ''pBad'' '''with RBS E0032''' <br> not in the <span style="color:#0000FF;">Core System</span>
+
|total transcription rate of <br> ''pBad'' '''with RBS E0032''' <br> ''not in the <span style="color:#0000FF;">Core System</span>''
|nM.min<sup>-1</sup>
|nM.min<sup>-1</sup>
|
|
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|-
|-
|(&gamma; K<sub>bad</sub>/''const.expr(pBad)'')
|(&gamma; K<sub>bad</sub>/''const.expr(pBad)'')
-
|activation constant of pBad <br> not in the system
+
|activation constant of ''pBad'' <br> ''not in the <span style="color:#0000FF;">Core System</span>''
|nM
|nM
|
|
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|-
|-
|n<sub>bad</sub>
|n<sub>bad</sub>
-
|complexation order of ''pBad''<br> not in the system
+
|complexation order of ''pBad''<br> ''not in the <span style="color:#0000FF;">Core System</span>''
|no dimension
|no dimension
|
|
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|-
|-
|K<sub>ara</sub>
|K<sub>ara</sub>
-
|complexation constant ''Arabinose><''AraC'' <br> not in the <span style="color:#0000FF;">Core System</span>
+
|complexation constant ''Arabinose''><''AraC'' <br> ''not in the <span style="color:#0000FF;">Core System</span>''
|nM
|nM
|
|
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|-
|-
|n<sub>ara</sub>
|n<sub>ara</sub>
-
|complexation order ''Arabinose><''AraC'' <br> not in the <span style="color:#0000FF;">Core System</span>
+
|complexation order ''Arabinose''><''AraC'' <br> ''not in the <span style="color:#0000FF;">Core System</span>''
|no dimension
|no dimension
|
|

Latest revision as of 13:01, 29 October 2008

param signification unit value comments
(fluorescence) value of the observed fluorescence au need for 20 measures with well choosen [arab]i
conversion conversion ratio between
fluorescence and concentration
↓ gives ↓ 		nM.au-1 (1/79.429)
[GFP] GFP concentration at steady-state nM
γGFP dilution-degradation rate
of GFP(mut3b)
↓ gives ↓ 		min-1 0.0198 Only Dilution
Time Cell Disvision : 35 min.
ƒ2 activity of
pBad with RBS E0032 		nM.min-1



param signification
corresponding parameters in the equations 		unit value comments
βbad total transcription rate of
pBad with RBS E0032
not in the Core System 		nM.min-1
(γ Kbad/const.expr(pBad)) activation constant of pBad
not in the Core System 		nM
nbad complexation order of pBad
not in the Core System 		no dimension The literature [?] gives nbad =
Kara complexation constant Arabinose><AraC
not in the Core System 		nM The literature [?] gives Kara =
nara complexation order Arabinose><AraC
not in the Core System 		no dimension The literature [?] gives nara =
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