Team:PennState/diauxie/progress

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<p>Test were then run to compare the level of induction between mixes of xylose, glucose and xylose, and glucose. The goal of this project is to obtain a noticeably higher level of induction when induced with xylose and glucose compared to the wildtype construct.</p>
<p>Test were then run to compare the level of induction between mixes of xylose, glucose and xylose, and glucose. The goal of this project is to obtain a noticeably higher level of induction when induced with xylose and glucose compared to the wildtype construct.</p>
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Revision as of 13:57, 29 October 2008

Home The Team The Project Parts Notebook

Diauxie Elimination

Introduction
The System
Strategies
Progress
Conclusions
Parts
References

NHR Biosensors

NHR Introduction
Phthalate Biosensor
BPA Biosensor
 Progress & Results

We have completed our first set of cloning phases and are conducting our first round of testing and modification. Each test construct (promoter + GFP) has been cloned into pSB1A2 and transformed into DH5α, W3110 ∆xylB-G, and W3110 ∆xylB-R. Preliminary induction studies were run to find the optimal induction time and to see in what range OD and fluorescence were linearly related.

Test Construct
[Test Consturct]
Promoter pSB1A2 DH5α W3110 ΔxylB-G W3110 ΔxylB-R
PN
P1
P3
P5

Test were then run to compare the level of induction between mixes of xylose, glucose and xylose, and glucose. The goal of this project is to obtain a noticeably higher level of induction when induced with xylose and glucose compared to the wildtype construct.

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