Team:MIT/Notebook

From 2008.igem.org

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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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{| style="color:#1b2c8a;background-color:#CCCCCC;" cellpadding="3" cellspacing="1" border="1" bordercolor="#green" width="62%" align="center"
!align="center"|[[Team:MIT|Home]]
!align="center"|[[Team:MIT|Home]]
!align="center"|[[Team:MIT/Team|The Team]]
!align="center"|[[Team:MIT/Team|The Team]]
!align="center"|[[Team:MIT/Project|The Project]]
!align="center"|[[Team:MIT/Project|The Project]]
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!align="center"|[[Team:MIT/Experiments|Experiments]]
!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:MIT/Modeling|Modeling]]
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!align="center"|[[Team:MIT/Modeling|Results]]
!align="center"|[[Team:MIT/Notebook|Notebook]]
!align="center"|[[Team:MIT/Notebook|Notebook]]
|}
|}
==Procedural Daily Notebook==
==Procedural Daily Notebook==
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*Please feel free to click on any given day in the calendar below to see what wet-lab procedures were conducted.
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*Click on any day below to see what wet-lab procedures were conducted.
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==Organizational Daily Notebook==  
==Organizational Daily Notebook==  
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*Please click [[Organizational Daily Notebook | here ]] for a detailed daily summary of our daily meetings, progress, discussions, and brainstorming.
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*Please see our [[team:MIT/Organizational_Daily_Notebook|daily notebook]] for a detailed daily summary of our daily meetings, brainstorming, discussions, and progress.
==Information==
==Information==
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* [[Team:MIT/sequences Primers/Plasmids/PCR sequences]]
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* [[Team:MIT/sequences|Primers/Plasmids/PCR sequences]]
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* [[Team:MIT/Lactobacillus|Lactobacillus delbrueckii subsp. bulgaricus Information & Protocols]]
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* [[Team:MIT/Vocabulary_List|Vocabulary List]]
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==Protocols==
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* [[Team:MIT/Lactobacillus Lactobacillus delbrueckii subsp. bulgaricus Information & Protocols]]
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*[http://openwetware.org/wiki/MIT_iGEM_T4_and_Quick_Ligation DNA Ligations]
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*[http://openwetware.org/wiki/MIT_iGEM_Top10_ChemComp_Ecoli_transformation_protocol#Regular_Transformation_protocol_for_Top10_cells Top10 chem competent cell transformation]
 +
*[http://openwetware.org/wiki/Knight:Electroporation BLR electrocompetent cell transformation]
 +
*[http://web.mit.edu/biopolymers/www/DNA.html DNA sequencing]
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*[http://openwetware.org/wiki/Endy_pcr PCR]
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*[http://openwetware.org/wiki/MIT_iGEM_Agarose_Gels Running DNA Gels]
 +
*[http://openwetware.org/wiki/MIT_iGEM_Restriction_Digests Restriction Digests]
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*[http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks Preparing Antibiotic Stocks]
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*[[Team:MIT/Protein_Purification|Protein Purification]]
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* [[Team:MIT/Tooth_binding_assay_protocol|Novel Tooth Binding Assay]]
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* [[Team:MIT/Transforming_Lactococcus lactis|Lactococcus lactis Transformation Protocol]]
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*[[Team:MIT/Colony_PCR|Colony PCR]]
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* [[Team:MIT/Tooth_binding_assay_protocol Tooth binding assay protocol]]
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== Data ==
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* [[Team:MIT/Vocabulary_List Vocabulary List]]
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* [[Team:MIT/Notebook/Data/toothbinding|Toothbinding assay]]
==Useful Links==
==Useful Links==
-
*[http://www.biology.utah.edu/jorgensen/wayned/ape/ |DNA Plasmid Editor]
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*[http://www.biology.utah.edu/jorgensen/wayned/ape/ DNA Plasmid Editor]
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*[http://slam.bs.jhmi.edu/gd/ |Peptide Sequence Manipulations]
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*[http://slam.bs.jhmi.edu/gd/ Peptide Sequence Manipulations]
*Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
*Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
*PMID 9920267<br style="clear:both;"/> A synthetic peptide adhesion epitope as a novel antimicrobial agent.<br style="clear:both;"/>
*PMID 9920267<br style="clear:both;"/> A synthetic peptide adhesion epitope as a novel antimicrobial agent.<br style="clear:both;"/>
*PMID 9062560<br style="clear:both;"/> Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.<br style="clear:both;"/>
*PMID 9062560<br style="clear:both;"/> Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.<br style="clear:both;"/>
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*PMID 12788739<br style="clear:both;"/> (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).<br style="clear:both;"/>
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*PMID 4055036<br style="clear:both;"/> Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles.<br style="clear:both;"/>
 +
*PMID 12788739<br style="clear:both;"/> Determination of the domain of the Lactobacillus delbrueckii subsp. bulgaricus cell surface proteinase PrtB involved in attachment to the cell wall after heterologous expression of the prtB gene in Lactococcus lactis. (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).<br style="clear:both;"/>
*PMID 11772607<br style="clear:both;"/> Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.
*PMID 11772607<br style="clear:both;"/> Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.
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*High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol [http://www.biomedcentral.com/1472-6750/7/15]
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
{| style="color:#1b2c8a;background-color:#CCCCCC;" cellpadding="3" cellspacing="1" border="1" bordercolor="#green" width="62%" align="center"
!align="center"|[[Team:MIT|Home]]
!align="center"|[[Team:MIT|Home]]
!align="center"|[[Team:MIT/Team|The Team]]
!align="center"|[[Team:MIT/Team|The Team]]
!align="center"|[[Team:MIT/Project|The Project]]
!align="center"|[[Team:MIT/Project|The Project]]
 +
!align="center"|[[Team:MIT/Experiments|Experiments]]
!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:MIT/Modeling|Modeling]]
!align="center"|[[Team:MIT/Modeling|Modeling]]
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Latest revision as of 14:25, 29 October 2008


Home The Team The Project Experiments Parts Submitted to the Registry Results Notebook

Procedural Daily Notebook

  • Click on any day below to see what wet-lab procedures were conducted.


Organizational Daily Notebook

  • Please see our daily notebook for a detailed daily summary of our daily meetings, brainstorming, discussions, and progress.

Information

Protocols

  • [http://openwetware.org/wiki/MIT_iGEM_T4_and_Quick_Ligation DNA Ligations]
  • [http://openwetware.org/wiki/MIT_iGEM_Top10_ChemComp_Ecoli_transformation_protocol#Regular_Transformation_protocol_for_Top10_cells Top10 chem competent cell transformation]
  • [http://openwetware.org/wiki/Knight:Electroporation BLR electrocompetent cell transformation]
  • [http://web.mit.edu/biopolymers/www/DNA.html DNA sequencing]
  • [http://openwetware.org/wiki/Endy_pcr PCR]
  • [http://openwetware.org/wiki/MIT_iGEM_Agarose_Gels Running DNA Gels]
  • [http://openwetware.org/wiki/MIT_iGEM_Restriction_Digests Restriction Digests]
  • [http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks Preparing Antibiotic Stocks]
  • Protein Purification
  • Novel Tooth Binding Assay
  • Lactococcus lactis Transformation Protocol
  • Colony PCR

Data

Useful Links

  • [http://www.biology.utah.edu/jorgensen/wayned/ape/ DNA Plasmid Editor]
  • [http://slam.bs.jhmi.edu/gd/ Peptide Sequence Manipulations]
  • Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
  • PMID 9920267
    A synthetic peptide adhesion epitope as a novel antimicrobial agent.
  • PMID 9062560
    Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
  • PMID 4055036
    Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles.
  • PMID 12788739
    Determination of the domain of the Lactobacillus delbrueckii subsp. bulgaricus cell surface proteinase PrtB involved in attachment to the cell wall after heterologous expression of the prtB gene in Lactococcus lactis. (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
  • PMID 11772607
    Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.
  • High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol [http://www.biomedcentral.com/1472-6750/7/15]




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