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Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)
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==Results and Discussion== | ==Results and Discussion== | ||
===Reaction temparature:37°C,09/12=== | ===Reaction temparature:37°C,09/12=== | ||
| - | + | ====センダーの培養液：1000μL、レシーバの培養液：1000μL==== | |
[[Image:Chiba_talks_JW1908_37_RS1_0912_01.gif|thumb|left|Fig. <br>12/09.E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.]] | [[Image:Chiba_talks_JW1908_37_RS1_0912_01.gif|thumb|left|Fig. <br>12/09.E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.]] | ||
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| + | ====センダーの培養液：100μL、レシーバの培養液：1000μL==== | ||
[[Image:Chiba_talks_JW1908_37_RS2_0912_01.gif|thumb|left|Fig. <br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 10.]] | [[Image:Chiba_talks_JW1908_37_RS2_0912_01.gif|thumb|left|Fig. <br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 10.]] | ||
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*センダーの培養液：10μL、レシーバの培養液：1000μL | *センダーの培養液：10μL、レシーバの培養液：1000μL | ||
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#CinI+LVAの培養液を混ぜた反応液は、GFPの蛍光強度が上昇しなかった。BBa_K084010が働いていないか、クロストークが起こっていないことが考えられる。 | #CinI+LVAの培養液を混ぜた反応液は、GFPの蛍光強度が上昇しなかった。BBa_K084010が働いていないか、クロストークが起こっていないことが考えられる。 | ||
# | # | ||
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===Reaction temparature:37°C=== | ===Reaction temparature:37°C=== | ||
====センダーの培養液：500μL、レシーバの培養液：500μL==== | ====センダーの培養液：500μL、レシーバの培養液：500μL==== | ||
Revision as of 14:29, 29 October 2008
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design
Sender
- [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI(BBa_K084007)]
- [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI(BBa_K084008)]
- [http://partsregistry.org/Part:BBa_K084009 plac+rbs+RhlI(LVA)(BBa_K084009)]
- [http://partsregistry.org/Part:BBa_K084010 plac+rbs+CinI(BBa_K0840010)]
- [http://partsregistry.org/Part:BBa_S03623 ptet+rbs+LuxI(LVA)(BBa_S03623)]
Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
Method
- Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 37°C or 30°C.
- Measured intensity of green fluorescence at regular time intervals.
Results and Discussion
Reaction temparature:37°C,09/12
センダーの培養液：1000μL、レシーバの培養液：1000μL
12/09.E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
センダーの培養液：100μL、レシーバの培養液：1000μL
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 10.
- センダーの培養液：10μL、レシーバの培養液：1000μL
- センダーの培養液：1μL、レシーバの培養液：1000μL
- CinI+LVAの培養液を混ぜた反応液は、GFPの蛍光強度が上昇しなかった。BBa_K084010が働いていないか、クロストークが起こっていないことが考えられる。
Reaction temparature:37°C
センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
センダーの培養液：100μL、レシーバの培養液：1000μL
Reaction temparature:30°C
センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
センダーの培養液：100μL、レシーバの培養液：1000μL
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 100.
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 10.
センダーの培養液：10μL、レシーバの培養液：1000μL
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 100.
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 100.
>Back to Sender experiment and result
