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TUDelft/23 September 2008
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{{Template:TUDelftiGEM2008_calendar}} | {{Template:TUDelftiGEM2008_calendar}} | ||
| - | =September 23rd | + | =September 23rd= |
==3A Assembly== | ==3A Assembly== | ||
| + | We're working further towards completion of K115030 and K115035 today by transforming the ligated K115022 and K115027 into commercial competent TOP10 (invitrogen). | ||
==Growing induced cultures== | ==Growing induced cultures== | ||
| - | We've started growing induced | + | We've started growing induced 30ºC and 37ºC cultures today for more luciferase activity measurements. Cultures grown contained either pSB1AT3, K115012, K115029, K115031, K115032, K115034 and K115036 (as it showed to be a good construct in the colony PCR below). Some had different plasmids, either pSB1AT3 or pSB1AK3, so we've grown them on Ampicillin to minimize growth differences. We might have to consider putting K115012 (the only one in pSB1AK3) in pSB1AT3 to make strains more similar. |
| - | We've also lysed cultures grown yesterday, as they were already around OD=0.6. We had planned all cultures to be finished somewhere at the same day, but our shaker could not get below | + | |
| + | We've also lysed cultures grown yesterday, as they were already around OD=0.6. We had planned all cultures to be finished somewhere at the same day, but our shaker could not get below 26ºC therefore the strains grew faster. | ||
==Colony PCR== | ==Colony PCR== | ||
| - | A | + | A colony PCR was performed on the K115032, K115033 and K115036 constructs. The results are displayed in figure 1. It was decided that 32 and 33 will be ligated and transformed again, colony A of K115036 will be used for miniprepping, transformation and future experimentation. |
| - | [[Image:TUDelftColonyPCR230908.jpg|thumb|center]] | + | [[Image:TUDelftColonyPCR230908.jpg|thumb|center|figure 1: Colony PCR of K115032, K115033 and K115036 transformations]] |
{{Template:TUDelftiGEM2008_sidebar}} | {{Template:TUDelftiGEM2008_sidebar}} | ||
Latest revision as of 15:40, 29 October 2008
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| [http://2008.igem.org/TUDelft/1_September_2008 1] | [http://2008.igem.org/TUDelft/2_September_2008 2] | [http://2008.igem.org/TUDelft/3_September_2008 3] | [http://2008.igem.org/TUDelft/4_September_2008 4] | [http://2008.igem.org/TUDelft/5_September_2008 5] | [http://2008.igem.org/wiki/index.php?title=TUDelft/6_September_2008&action=edit 6] | [http://2008.igem.org/wiki/index.php?title=TUDelft/7_September_2008&action=edit 7] |
| [http://2008.igem.org/TUDelft/8_September_2008 8] | [http://2008.igem.org/TUDelft/9_September_2008 9] | [http://2008.igem.org/TUDelft/10_September_2008 10] | [http://2008.igem.org/TUDelft/11_September_2008 11] | [http://2008.igem.org/TUDelft/12_September_2008 12] | [http://2008.igem.org/wiki/index.php?title=TUDelft/13_September_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=TUDelft/14_September_2008&action=edit 14] |
| [http://2008.igem.org/TUDelft/15_September_2008 15] | [http://2008.igem.org/TUDelft/16_September_2008 16] | [http://2008.igem.org/TUDelft/17_September_2008 17] | [http://2008.igem.org/TUDelft/18_September_2008 18] | [http://2008.igem.org/TUDelft/19_September_2008 19] | [http://2008.igem.org/wiki/index.php?title=TUDelft/20_September_2008&action=edit 20] | [http://2008.igem.org/wiki/index.php?title=TUDelft/21_September_2008&action=edit 21] |
| [http://2008.igem.org/TUDelft/22_September_2008 22] | [http://2008.igem.org/TUDelft/23_September_2008 23] | [http://2008.igem.org/TUDelft/24_September_2008 24] | [http://2008.igem.org/TUDelft/25_September_2008 25] | [http://2008.igem.org/wiki/index.php?title=TUDelft/26_September_2008&action=edit 26] | [http://2008.igem.org/wiki/index.php?title=TUDelft/27_September_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=TUDelft/28_September_2008&action=edit 28] |
| [http://2008.igem.org/TUDelft/29_September_2008 29] | [http://2008.igem.org/TUDelft/30_September_2008 30] | |||||
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| [http://2008.igem.org/TUDelft/1_October_2008 1] | [http://2008.igem.org/TUDelft/2_October_2008 2] | [http://2008.igem.org/TUDelft/3_October_2008 3] | [http://2008.igem.org/wiki/index.php?title=TUDelft/4_October_2008&action=edit 4] | [http://2008.igem.org/wiki/index.php?title=TUDelft/5_October_2008&action=edit 5] | ||
| [http://2008.igem.org/TUDelft/6_October_2008 6] | [http://2008.igem.org/TUDelft/7_October_2008 7] | [http://2008.igem.org/TUDelft/8_October_2008 8] | [http://2008.igem.org/TUDelft/9_October_2008 9] | [http://2008.igem.org/TUDelft/10_October_2008 10] | [http://2008.igem.org/wiki/index.php?title=TUDelft/11_October_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=TUDelft/12_October_2008&action=edit 12] |
| [http://2008.igem.org/TUDelft/13_October_2008 13] | [http://2008.igem.org/TUDelft/14_October_2008 14] | [http://2008.igem.org/TUDelft/15_October_2008 15] | [http://2008.igem.org/TUDelft/16_October_2008 16] | [http://2008.igem.org/TUDelft/17_October_2008 17] | [http://2008.igem.org/wiki/index.php?title=TUDelft/18_October_2008&action=edit 18] | [http://2008.igem.org/wiki/index.php?title=TUDelft/19_October_2008&action=edit 19] |
| [http://2008.igem.org/TUDelft/20_October_2008 20] | [http://2008.igem.org/TUDelft/21_October_2008 21] | [http://2008.igem.org/TUDelft/22_October_2008 22] | [http://2008.igem.org/TUDelft/23_October_2008 23] | [http://2008.igem.org/TUDelft/24_October_2008 24] | [http://2008.igem.org/wiki/index.php?title=TUDelft/25_October_2008&action=edit 25] | [http://2008.igem.org/wiki/index.php?title=TUDelft/26_October_2008&action=edit 26] |
| [http://2008.igem.org/wiki/index.php?title=TUDelft/27_October_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=TUDelft/28_October_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=TUDelft/29_October_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=TUDelft/30_October_2008&action=edit 30] | [http://2008.igem.org/wiki/index.php?title=TUDelft/31_October_2008&action=edit 31] | ||
Contents |
September 23rd
3A Assembly
We're working further towards completion of K115030 and K115035 today by transforming the ligated K115022 and K115027 into commercial competent TOP10 (invitrogen).
Growing induced cultures
We've started growing induced 30ºC and 37ºC cultures today for more luciferase activity measurements. Cultures grown contained either pSB1AT3, K115012, K115029, K115031, K115032, K115034 and K115036 (as it showed to be a good construct in the colony PCR below). Some had different plasmids, either pSB1AT3 or pSB1AK3, so we've grown them on Ampicillin to minimize growth differences. We might have to consider putting K115012 (the only one in pSB1AK3) in pSB1AT3 to make strains more similar.
We've also lysed cultures grown yesterday, as they were already around OD=0.6. We had planned all cultures to be finished somewhere at the same day, but our shaker could not get below 26ºC therefore the strains grew faster.
Colony PCR
A colony PCR was performed on the K115032, K115033 and K115036 constructs. The results are displayed in figure 1. It was decided that 32 and 33 will be ligated and transformed again, colony A of K115036 will be used for miniprepping, transformation and future experimentation.
