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Imperial College/7 October 2008
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| - | + | =7 October 2008= | |
| - | == | + | ==Wet Lab== |
*XL1 Blue Competent cells were prepared today. Three tests were carried out on the last three aliquots of competent cells. One aliquot was transformed with DNA and two were transformed with no DNA and plated on Amp and Kan. Only the cells transformed with DNA grew, showing no contamination was present. | *XL1 Blue Competent cells were prepared today. Three tests were carried out on the last three aliquots of competent cells. One aliquot was transformed with DNA and two were transformed with no DNA and plated on Amp and Kan. Only the cells transformed with DNA grew, showing no contamination was present. | ||
| - | *Digests of Midi preps for the 5'EpsE, 3'EpsE and Spectinomycin resistance gene. Two types of digestion were performed: i) first EcoRI and PstI to check the part size is correct,(bottom gel) ii) XbaI and SpeI to chek the correct orientation of the insert(top gel). | + | *Digests of Midi preps for the 5'EpsE, 3'EpsE and Spectinomycin resistance gene. Two types of digestion were performed: i) first EcoRI and PstI to check the part size is correct,(bottom gel) ii) XbaI and SpeI to chek the correct orientation of the insert (top gel). |
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[[Image:Dig9th.PNG|center|500px]] | [[Image:Dig9th.PNG|center|500px]] | ||
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*As i5 can be seen EpsE 3'and Spec all have correct sized and orientated insert, these will now be sequenced. The EpsE5' does have the correctly sized insert but the orientation is incorrect. Mini preps will be prepared for the EpsE 5' to obtain a correctly orientated insert. | *As i5 can be seen EpsE 3'and Spec all have correct sized and orientated insert, these will now be sequenced. The EpsE5' does have the correctly sized insert but the orientation is incorrect. Mini preps will be prepared for the EpsE 5' to obtain a correctly orientated insert. | ||
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Latest revision as of 17:56, 29 October 2008
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7 October 2008Wet Lab
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