"
Team:UC Berkeley/Resources/MMCalculator
From 2008.igem.org
(Difference between revisions)
| Line 63: | Line 63: | ||
out.value += "Ligation Buffer: " + (1*num) + "\n"; | out.value += "Ligation Buffer: " + (1*num) + "\n"; | ||
out.value += "T4 Ligase: " + (.5*num) + "\n"; | out.value += "T4 Ligase: " + (.5*num) + "\n"; | ||
| + | out.value += "Digested pBca1256 Vector: " + (1*num) + "\n"; | ||
| + | out.value += "Aliquot 9 uL\n"; | ||
| + | break; | ||
| + | case 6: | ||
| + | out.value += "Regular PCR w/o DMSO, 50 uL Scale Master Mix for " + num + " reactions:\n"; | ||
| + | out.value += "Water: " + (43*num) + "\n"; | ||
| + | out.value += "10 mM dNTP: " + (1*num) + "\n"; | ||
| + | out.value += "10x Expand Buffer: " + (.5*num) + "\n"; | ||
out.value += "Digested pBca1256 Vector: " + (1*num) + "\n"; | out.value += "Digested pBca1256 Vector: " + (1*num) + "\n"; | ||
out.value += "Aliquot 9 uL\n"; | out.value += "Aliquot 9 uL\n"; | ||
| Line 81: | Line 89: | ||
<option>Digestion</option> | <option>Digestion</option> | ||
<option>Ligation</option> | <option>Ligation</option> | ||
| + | <option>Regular PCR w/o DMSO, 50 uL scale</option> | ||
</select> | </select> | ||
<br> | <br> | ||
Revision as of 18:40, 26 June 2008
Bing's Master Mix Calcualtor Version 0.1a
All volumes are given in uL. The calculator will give you enough master mix for one extra reaction.
