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Team:UC Berkeley/Resources/MMCalculator
From 2008.igem.org
(Difference between revisions)
| Line 40: | Line 40: | ||
case 3: | case 3: | ||
out.value += "Colony PCR Master Mix for " + num + " reactions:\n"; | out.value += "Colony PCR Master Mix for " + num + " reactions:\n"; | ||
| - | out.value += "Water: " + ( | + | out.value += "Water: " + (16.1*num) + "\n"; |
| - | out.value += "MgCl ( | + | out.value += "MgCl (50 mM): " + (.6*num) + "\n"; |
| - | out.value += "10 mM dNTP: " + ( | + | out.value += "10 mM dNTP: " + (.4*num) + "\n"; |
out.value += "10x Taq Buffer: " + (2*num) + "\n"; | out.value += "10x Taq Buffer: " + (2*num) + "\n"; | ||
| - | out.value += "Taq Polymerase: " + (. | + | out.value += "Taq Polymerase: " + (.1*num) + "\n"; |
out.value += "ca998 Oligo(10 mM): " + (.4*num) + "\n"; | out.value += "ca998 Oligo(10 mM): " + (.4*num) + "\n"; | ||
out.value += "g00101 Oligo(10 mM): " + (.4*num) + "\n"; | out.value += "g00101 Oligo(10 mM): " + (.4*num) + "\n"; | ||
Revision as of 19:53, 26 June 2008
Bing's Master Mix Calcualtor Version 0.1a
All volumes are given in uL. The calculator will give you enough master mix for one extra reaction.
