Alberta NINT/24 June 2008

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Line 8: Line 8:
             Made overnights of the colonies from 23/06/08 transformation.
             Made overnights of the colonies from 23/06/08 transformation.
             Made glycerol stock of K102002.
             Made glycerol stock of K102002.
 +
 +
lab work (JD):
 +
              Digested K102003 with BamHI and NcoI and incubated for 2 hrs at 37C
 +
              Ran gel slowly on 2% agarose
 +
              Cut out desired fragment and gel purified
 +
              Counted and recorded colonies from yesterdays ligation:
 +
                              Negative        200
 +
                              K102007        200
 +
              From results it seems ligation didn't work.
 +
              Quantified K102003/B+N.
 +
 +
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Revision as of 21:03, 26 June 2008

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lab work (SD): 

            Transformation results: 2 colonies on K102008 plate and 2 colonies on K102008 negative control plate
            Redid ligation of K102002/B+N with TA0/B+N (1/100) and transformation into XL1-B cells.
            Made overnights of the colonies from 23/06/08 transformation.
            Made glycerol stock of K102002.

lab work (JD): 

             Digested K102003 with BamHI and NcoI and incubated for 2 hrs at 37C
             Ran gel slowly on 2% agarose
             Cut out desired fragment and gel purified
             Counted and recorded colonies from yesterdays ligation:
                             Negative         200
                             K102007        200
              From results it seems ligation didn't work.
              Quantified K102003/B+N.

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