Team:Chiba/Calendar-Home/16 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Laboratory work)
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===Team:Communication===
===Team:Communication===
[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] with (15/9) cultures.
[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] with (15/9) cultures.
-
*reaction temparaturewas 37°C and 30°C.)
+
*reaction temparaturewas 37°C and 30°C.)
*result
*result
Line 16: Line 16:
===Team:Output===
===Team:Output===
<br>Digestion test
<br>Digestion test
-
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(x2)
+
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(&times;2)
-
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(x5)
+
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(&times;5)
-
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(x2)
+
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(&times;2)
*[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
*[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
*[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
*[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
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</tr>
</tr>
<tr>
<tr>
-
<td>DNA(μL)</td>
+
<td>DNA(&mu;L)</td>
<td>3</td>
<td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ(μL)</td>
+
<td>EcoRI(&mu;L)</td>
<td>0.083</td>
<td>0.083</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ(μL)</td>
+
<td>PstI(&mu;L)</td>
<td>0.083</td>
<td>0.083</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA(x10)(μL)</td>
+
<td>BSA(&times;10)(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer3(x10)(μL)</td>
+
<td>Buffer3(&times;10)(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O(μL)</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
</table>
</table>
-
-->37℃ 1hour
+
-->37&deg;C 1 hour
[[Team:Chiba/protocol/gelcheck|Gel check]]
[[Team:Chiba/protocol/gelcheck|Gel check]]
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</tr>
</tr>
<tr>
<tr>
-
<td>DNA(μL)</td>
+
<td>DNA(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye(μL)</td>
+
<td>Loading Dye(&mu;L)</td>
<td>2</td>
<td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>12</td>
<td>12</td>
</tr>
</tr>
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</tr>
</tr>
<tr>
<tr>
-
<td>DNA(μL)</td>
+
<td>DNA(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Venus Fwd(μL)</td>
+
<td>Venus Fwd(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Rev(VR)(μL)</td>
+
<td>Rev(VR)(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Thermo pol Buffer(μL)</td>
+
<td>Thermo pol Buffer(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dNTP mix(μL)</td>
+
<td>dNTP mix(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Vent(μL)</td>
+
<td>Vent(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O(μL)</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>68</td>
<td>68</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>100</td>
<td>100</td>
</tr>
</tr>
</table>
</table>

Revision as of 19:46, 29 October 2008

>Home | Notebook

15 September 2008 <|> 17 September 2008

Laboratory work

Team:Communication

time-delay test with (15/9) cultures.

  • reaction temparaturewas 37°C and 30°C.)
  • result


  • Created Glycerol Stocks from (15/9) cultures.

Team:Output


Digestion test

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(×2)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(×5)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(×2)
  • [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
  • [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
  • [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)
Sample No. each
DNA(μL) 3
EcoRI(μL) 0.083
PstI(μL) 0.083
BSA(×10)(μL) 1
Buffer3(×10)(μL) 1
dH2O(μL) 5
TOTAL(μL) 10

-->37°C 1 hour

Gel check

Sample No. each
DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12

Transformation

  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

PCR

  • Venus YFP(ptet+LuxR+YFP)
Sample No. YFP
DNA(μL) 1
Venus Fwd(μL) 5
Rev(VR)(μL) 5
Thermo pol Buffer(μL) 10
dNTP mix(μL) 10
Vent(μL) 1
dH2O(μL) 68
TOTAL(μL) 100
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