Team:Chiba/Calendar-Home/17 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
Line 13: Line 13:
***[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
***[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
**E.coli strain<br>Senders:JW1908<br>Receiver:JW1908<br>
**E.coli strain<br>Senders:JW1908<br>Receiver:JW1908<br>
-
**reaction temparature<br>37°C and 30°C
+
**reaction temparature<br>37&deg;C and 30&deg;C
Line 22: Line 22:
***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
-
**reaction temparature<br>37°C and 30°C
+
**reaction temparature<br>37&deg;C and 30&deg;C
===Team:Output===
===Team:Output===
Line 49: Line 49:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA template(μL)</td>
+
<td>DNA template(&mu;L)</td>
<td>25</td>
<td>25</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ(μL)</td>
+
<td>EcoRI(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ(μL)</td>
+
<td>PstI(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA(x10)(μL)</td>
+
<td>BSA(&times;10)(&mu;L)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ Buffer(μL)</td>
+
<td>EcoRI Buffer(&mu;L)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O(μL)</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>40</td>
<td>40</td>
</tr>
</tr>
</table>
</table>
-
-->Left at37 degrees for 1 hour.
+
-->Left at 37 &deg;C for 1 hour.
-->Next day
-->Next day

Revision as of 19:48, 29 October 2008

>Home | Notebook

16 September 2008 <|> 18 September 2008

Laboratory work

Team:Communication

  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
      • [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
    • E.coli strain
      Senders:JW1908
      Receiver:JW1908
    • reaction temparature
      37°C and 30°C


  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
    • E.coli strain
      Senders:XL10GOLD
      Receiver:JW1908
    • reaction temparature
      37°C and 30°C

Team:Output

Transformed

  • [http://partsregistry.org/Part:BBa_I13500 BBa_I13500](RBS+GFP)
  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](Luxpr+LuxR)-->no colony

miniprepped

  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](Luxpr+LuxR+LuxR+GFP)
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

Digestion

  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](ptetR+RBS)
  • [http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
  • Ligation product(ptet+LasR)
  • Ligation product(ptet+RhlR)
  • Ligation product(ptet+cinR)
Sample No. each
DNA template(μL) 25
EcoRI(μL) 1
PstI(μL) 1
BSA(×10)(μL) 4
EcoRI Buffer(μL) 4
dH2O(μL) 5
TOTAL(μL) 40

-->Left at 37 °C for 1 hour.

-->Next day

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