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User:University of Washington/26 June 2008
From 2008.igem.org
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| - | -Obtained yeast growth media | + | -Obtained yeast growth media YEPD |
-Obtained yeast transforming media 50% PEG, 10x LiAc, and 10x TE | -Obtained yeast transforming media 50% PEG, 10x LiAc, and 10x TE | ||
| + | |||
| + | -Inoculated isolated yeast colonies in 5 mL of YEPD media and incubated at 30 degrees Celsius in a rotator | ||
== Conjugation Plasmid == | == Conjugation Plasmid == | ||
Revision as of 22:30, 26 June 2008
Yeast Shuttle Plasmid
-Obtained yeast growth media YEPD
-Obtained yeast transforming media 50% PEG, 10x LiAc, and 10x TE
-Inoculated isolated yeast colonies in 5 mL of YEPD media and incubated at 30 degrees Celsius in a rotator
Conjugation Plasmid
Mini Preps
·C600+RP4 construct
Glycerol Stocks
·C600+RP4
·S17-1
LuxR from AraC and TetR
- Got 1 colony for TetR, 24 for AraC
- Grew culture for Glycerol Stock and Miniprep
Team:University_of_Washington/Notebook#Notebook
