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User:University of Washington/25 June 2008
From 2008.igem.org
(Difference between revisions)
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-transformed and plated with Amp | -transformed and plated with Amp | ||
| - | == LuxR under | + | == LuxR under pLac == |
-Obtained parts R0010, I0462, and J04430. | -Obtained parts R0010, I0462, and J04430. | ||
Revision as of 22:40, 26 June 2008
Yeast Shuttle Plasmid
-Obtained plate of yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0)
-Incubated yeast at 30 degrees Celsius overnight
LuxR under AraC and TetR
-Obtained BBa_R0040 (TetR), BBa_R0080 (AraC)
-transformed and plated with Amp
LuxR under pLac
-Obtained parts R0010, I0462, and J04430. -R0010 and I0462 were transformed into MG strains, and plated on Amp. -J04430 was transformed into DH strain, and plated on Amp.
Team:University_of_Washington/Notebook#Notebook
