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Team:Imperial College/Protocols
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| - | {{Imperial/EndPage| | + | === Experiments & Protocols === |
| + | {{Imperial/Box2|| | ||
| + | Below you will find a comprehensive list of all the protocols we used during our wet lab work. We spent some time toward the start of the project sourcing and updating effective protocols for ''B. subtilis'' manipulation explicitly, as other teams have previously had difficulty in this area. | ||
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| + | These protocols worked for us, so any future teams looking to use ''subtilis'' could save quite a bit of time by looking here before diving into the literature!}} | ||
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| + | {{Imperial/Box1|Protocols| | ||
| + | ====== 1. Transformation Protocols for integration vectors in ''Bacillus subtilis'' ====== | ||
| + | *[[Team:Imperial College/Transformation |Protocol 1]] | ||
| + | *[[Team:Imperial College/Transformation_2 |Protocol 2]] | ||
| + | ====== 2. Transformation Protocol for ''E.coli'' ====== | ||
| + | *[[Team:Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]] | ||
| + | ====== 3. PCR ====== | ||
| + | *[[Team:Imperial College/PCR |PCR]] | ||
| + | ====== 4. Calibration Curves ====== | ||
| + | *[[Team:Imperial College/Calibration_Curves |Calibration Curves ]] | ||
| + | *[[Team:Imperial College/Growth |O.D <sub>600</sub> vs cell number]] | ||
| + | *[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]] | ||
| + | ====== 5. Promoter-RBS Testing Protocols ====== | ||
| + | *[[Team:Imperial College/Constitutive |Constitutive Promoters and RBS]] | ||
| + | *[[Team:Imperial College/Inducible |pHyperspank Promoter and RBS]] | ||
| + | *[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]] | ||
| + | ====== 6. Antibiotic Resistance Testing ====== | ||
| + | *[[Team:Imperial College/Resistance |Chloramphenicol Resistance Assay]] | ||
| + | |}} | ||
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| + | <br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}} | ||
Latest revision as of 21:14, 29 October 2008
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Experiments & Protocols
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