Team:Imperial College/Protocols

From 2008.igem.org

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=== Experiments & Protocols ===
=== Experiments & Protocols ===
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These protocols worked for us, so any future teams looking to use ''subtilis'' could save quite a bit of time by looking here before diving into the literature!}}
These protocols worked for us, so any future teams looking to use ''subtilis'' could save quite a bit of time by looking here before diving into the literature!}}
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{| cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="center" width=50%
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{{Imperial/Box1|Protocols|
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|width="100%"| <font size=4px color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''</font>
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====== 1. Transformation Protocols for integration vectors in ''Bacillus subtilis'' ======
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*[[Team:Imperial  College/Transformation |Protocol 1]]
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*[[Team:Imperial  College/Transformation_2 |Protocol 2]]
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*[[/Transformation_protocol_2 | Protocol 1]]
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====== 2. Transformation Protocol for ''E.coli'' ======
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*[[/Transformation_protocol_4 | Protocol 2]]
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*[[Team:Imperial  College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]
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====== 3. PCR ======
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|style="background:#66aadd"| <font size=4px color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''</font>
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*[[Team:Imperial  College/PCR |PCR]]
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====== 4. Calibration Curves ======
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*[[Team:Imperial  College/Calibration_Curves |Calibration Curves ]]
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*Protocol
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*[[Team:Imperial  College/Growth |O.D <sub>600</sub> vs cell number]]
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*[[Team:Imperial  College/Fluorescence |Fluorescence vs GFPmut3b]]
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|style="background:#66aadd"| <font size=4px color=white>'''3. Transformation Protocol ''E.coli'''''</font>
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====== 5. Promoter-RBS Testing Protocols ======
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*[[Team:Imperial  College/Constitutive |Constitutive Promoters and RBS]]  
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*[[Team:Imperial  College/Inducible |pHyperspank Promoter and RBS]]
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*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]
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*[[Team:Imperial  College/Light_Inducible |Light Inducible Promoters and RBS]]
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====== 6. Antibiotic Resistance Testing  ======
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|style="background:#66aadd"| <font size=4px color=white>'''4. Motility Analysis'''</font>
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*[[Team:Imperial  College/Resistance |Chloramphenicol Resistance Assay]]
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*[[/Protocols#Motility_Experiments | Motility Assays]]
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|style="background:#66aadd"| <font size=4px color=white>'''5. PCR'''</font>
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*[[/PCR| PCR]]
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*[[/Colony_PCR| Single Colony PCR]]
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|style="background:#66aadd"| <font size=4px color=white>'''6. Calibration Curves'''</font>
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*[[/Calibration-Curves| Calibration Curves ]]
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*[[/Growth| O.D.<sub>600</sub> vs cell number]]
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*[[/Fluorescence| Fluorescence vs GFPmut3b]]
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|style="background:#66aadd"| <font size=4px color=white>'''7. Promoter-RBS Testing Protocols'''</font>
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*[[/Constitutive | Constitutive Promoters and RBS]]  
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*[[/Inducible | pHyperspank Promoter and RBS]]
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*[[/Light_Inducible | Light Inducible Promoters and RBS]]
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|style="background:#66aadd"| <font size=4px color=white>'''8. Biomaterial Protocols'''</font>
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*Protocol
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<br><hr>{{Imperial/EndPage|Cloning_Strategy|Major_Results}}
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<br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}

Latest revision as of 21:14, 29 October 2008


Experiments & Protocols

Below you will find a comprehensive list of all the protocols we used during our wet lab work. We spent some time toward the start of the project sourcing and updating effective protocols for B. subtilis manipulation explicitly, as other teams have previously had difficulty in this area.

These protocols worked for us, so any future teams looking to use subtilis could save quite a bit of time by looking here before diving into the literature!


Protocols
1. Transformation Protocols for integration vectors in Bacillus subtilis
2. Transformation Protocol for E.coli
3. PCR
4. Calibration Curves
5. Promoter-RBS Testing Protocols
6. Antibiotic Resistance Testing



Retrieved from "http://2008.igem.org/Team:Imperial_College/Protocols"