Team:Imperial College/Protocols

From 2008.igem.org

(Difference between revisions)

Latest revision as of 21:14, 29 October 2008

Experiments & Protocols

Below you will find a comprehensive list of all the protocols we used during our wet lab work. We spent some time toward the start of the project sourcing and updating effective protocols for B. subtilis manipulation explicitly, as other teams have previously had difficulty in this area.

These protocols worked for us, so any future teams looking to use subtilis could save quite a bit of time by looking here before diving into the literature!

Protocols

1. Transformation Protocols for integration vectors in Bacillus subtilis

2. Transformation Protocol for E.coli

Preparation of XL1-blue electrocompetent cells

3. PCR

PCR

4. Calibration Curves

5. Promoter-RBS Testing Protocols

6. Antibiotic Resistance Testing

Chloramphenicol Resistance Assay

< Previous

Back to top

Next >

@@ Line 11: / Line 11: @@
 *[[Team:Imperial  College/Transformation |Protocol 1]]
 *[[Team:Imperial  College/Transformation_2 |Protocol 2]]
-====== 2. Transformation Protocol for linear DNA in ''Bacillus subtilis'' ======
+====== 2. Transformation Protocol for ''E.coli'' ======
-*[[Team:Imperial  College/Transformation_3 |Protocol]]
-====== 3. Transformation Protocol for ''E.coli'' ======
 *[[Team:Imperial  College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]
-====== 4. PCR ======
+====== 3. PCR ======
 *[[Team:Imperial  College/PCR |PCR]]
-*[[Team:Imperial  College/SCPCR |Single Colony PCR]]
+====== 4. Calibration Curves ======
-====== 5. Calibration Curves ======
 *[[Team:Imperial  College/Calibration_Curves |Calibration Curves ]]
 *[[Team:Imperial  College/Growth |O.D <sub>600</sub> vs cell number]]
 *[[Team:Imperial  College/Fluorescence |Fluorescence vs GFPmut3b]]
-====== 6. Promoter-RBS Testing Protocols ======
+====== 5. Promoter-RBS Testing Protocols ======
 *[[Team:Imperial  College/Constitutive |Constitutive Promoters and RBS]]
 *[[Team:Imperial  College/Inducible |pHyperspank Promoter and RBS]]
 *[[Team:Imperial  College/Light_Inducible |Light Inducible Promoters and RBS]]
-====== 7. Biomaterial Protocols ======
+====== 6. Antibiotic Resistance Testing  ======
-*[[Team:Imperial  College/Biomaterial_Assay |Biomaterial Assay]]
+*[[Team:Imperial  College/Resistance |Chloramphenicol Resistance Assay]]
 |}}
 <br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}