Team:Warsaw

From 2008.igem.org

(Difference between revisions)
 
(44 intermediate revisions not shown)
Line 1: Line 1:
-
<html xmlns="http://www.w3.org/1999/xhtml">
+
{{WarHead|headimg=2008.igem.org/wiki/images/f/fa/Logo_home.jpg}}
-
<head>
+
<html>
-
<title>igrm_home.jpg</title>
+
-
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-2" />
+
-
<link href="igem.css" rel="stylesheet" type="text/css" />
+
<h1>A novel system for generation and screening for new interactors targeted against any bait</h1>
-
<style>
+
<p><ul>
-
p {
+
<li>maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter</li><li>
-
font-family: Georgia, "Times New Roman", Times, serif;
+
interactor presentation on bacterial surface</li><li>
-
text-align: justify;
+
selection of best bait interactor</li>
-
}
+
</ul></p>
-
body {
+
<br>
-
margin: 0px;
+
-
}
+
-
h1 {
+
-
font-family: Georgia, "Times New Roman", Times, serif;
+
-
font-size: 14pt;
+
-
letter-spacing: 14pt;
+
-
margin-top: 14pt;
+
-
color: #070D3F;
+
-
}
+
-
.home {
+
-
font-family: Georgia, "Times New Roman", Times, serif;
+
-
font-size: 12pt;
+
-
font-style: italic;
+
-
margin-right: 1cm;
+
-
margin-left: 1cm;
+
-
padding-right: 1cm;
+
-
padding-left: 1cm;
+
-
text-align: justify;
+
-
text-indent: 2em;
+
-
padding-top: 20pt;
+
-
}
+
-
td img {display: block;}
+
<div align="center"><img src="https://static.igem.org/mediawiki/2008/6/6a/Krag_zycia2.gif" usemap="#Map"/>
-
h2 {
+
<map name="Map" id="Map">
-
font-family: Georgia, "Times New Roman", Times, serif;
+
  <area shape="poly" coords="596,397,458,396,454,334,598,331,593,337" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#selection" />
-
font-size: 14pt;
+
<area shape="poly" coords="346,434,234,422,154,661,337,666" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#mutagenesis" />
-
font-weight: bold;
+
<area shape="poly" coords="210,265,278,225,323,227,372,250,376,254,582,53,534,5,395,1,6,1,30,157" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#hunter" />
-
color: #1F2E65;
+
<area shape="poly" coords="185,349,211,388,23,470,9,385" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#selection" />
-
margin-bottom: 0px;
+
<area shape="circle" coords="300,329,98" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm" />
-
padding-bottom: 0px;
+
</map>
-
}
+
</div>
-
li {
+
</td>
-
font-family: Georgia, "Times New Roman", Times, serif;
+
  <td></td>
-
text-align: justify;
+
  </tr>
-
}
+
<tr><td>
-
h3 {
+
<h3>Institutions whom we would like to thank for financial and technical support:</h3>
-
font-family: Georgia, "Times New Roman", Times, serif;
+
<table width="100%" border="0" cellspacing="10" cellpadding="0" align="center">
-
font-size: 12pt;
+
-
font-weight: bold;
+
-
color: #0C1347;
+
-
}
+
-
.ods {
+
-
padding-right: 20pt;
+
-
padding-left: 20pt;
+
-
}
+
-
</style>
+
-
</head>
+
-
 
+
-
 
+
-
<body bgcolor="#ffffff">
+
-
<table width="800" border="0" align="center" cellpadding="0" cellspacing="0">
+
-
<!-- fwtable fwsrc="https://static.igem.org/mediawiki/2008/7/7e/Str_warszawa_tlo_slice.png" fwbase="igrm_home.jpg" fwstyle="Dreamweaver" fwdocid = "1333815265" fwnested="0" -->
+
   <tr>
   <tr>
-
  <td><img src="spacer.gif" width="800" height="1" border="0" alt="" /></td>
+
    <td align="center" valign="bottom"><a href="http://www.uw.edu.pl/en/"><img src="https://static.igem.org/mediawiki/2008/6/67/Uw.gif" width="100" height="99" border="0" /></a>
-
  <td><img src="spacer.gif" width="1" height="1" border="0" alt="" /></td>
+
    <a href="http://www.uw.edu.pl/en/"><h4>University of Warsaw</h4> </a></td>
 +
    <td align="center" valign="bottom">
 +
<a href="http://www.biol.uw.edu.pl/_2008eng/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="57" height="75" border="0" /></a>
 +
    <a href="http://www.biol.uw.edu.pl/_2008eng/"><h4>Faculty of Biology, University of Warsaw </h4></a>     
 +
</td>
   </tr>
   </tr>
 +
  <tr>
 +
    <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a>
 +
    <a href="http://www.fundacjauv.org.pl/"><h4>The Universitatis Varsoviensis Foundation </h4></a></td>
 +
    <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="221" height="75" border="0" /></a>
 +
    <a href="http://www.fnp.org.pl/ang/index.html"><h4>Foundation for Polish Science </h4></a></td>
 +
  </tr>
 +
  <tr>
 +
    <td align="center" valign="bottom"><a href="http://oligo.pl/English/"><img src="https://static.igem.org/mediawiki/2008/8/8e/Oligo.gif" width="183" height="64" border="0" /></a>    </td>
 +
    <td align="center" valign="bottom"><img src="https://static.igem.org/mediawiki/2008/6/60/Rada_Konsultacyjna.gif" width="160" height="120" border="0" /></td>
 +
  </tr>
 +
  <tr>
 +
    <td align="center" valign="bottom">
 +
<a href="http://www.fuw.pl"><h4>Warsaw University Foundation</h4></a>
 +
</td>
 +
    <td align="center" valign="bottom">
 +
<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h4>Department of Molecular Biology, International Institute of Molecular and Cell Biology</h4></a>
 +
</td>
 +
  </tr>
 +
 +
<tr>
 +
<td colspan="2" align="center" valign="bottom">
 +
<p>Units belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
 +
</td>
   <tr>
   <tr>
-
  <td><img name="igrm_home_r1_c1" src="igrm_home_r1_c1.jpg" width="800" height="270" border="0" id="igrm_home_r1_c1" usemap="#m_igrm_home_r1_c1" alt="" /></td>
+
    <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h4>Department of Plant Molecular Biology </h4></a></td>
-
  <td><img src="spacer.gif" width="1" height="270" border="0" alt="" /></td>
+
    <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h4>Department of Virology</h4> </a> </td>
   </tr>
   </tr>
   <tr>
   <tr>
-
  <td valign="top" background="igrm_notebook_r2_c1.jpg" class="home">Na początku należy uprzedzić że nasze pierwsze plany w pewnej mierze rozminęły się z tym co w końcu zrobiliśmy. A plany mieliśmy naprawdę niesamowite: chcieliśmy stworzyć układ maszyn biologicznych, które umożliwiały badanie oddziaływań białek przy jednoczesnej próbie zmiany sekwencji jednego z nich tak aby znaleźć najsilniejsze możliwe oddziaływanie. Cały układ miał wyglądać mniej więcej następująco. Sekwencja DNA badanego białka lub cała biblioteka takich sekwencji umieszczona na nisko kopijnym plazmidzie miała zostać transformowana do szczepu w którym ta sekwencja ulegałaby stosunkowo częstym mutacjom (nazwijmy ten szczep "jednorękim bandytą"). Jednocześnie plazmid ten umożliwiałby prezentację tychże białek na powierzchni komórek bakteryjnych gdzie istniałaby możliwość selekcji komórek posiadających białko najsilniej oddziałujące z białkiem wprowadzonym do pożywki. Wyobrażaliśmy sobie że taki układ urządzeń pozwoliłby na znajdowanie przeciwciał o nowych właściwościach czy przeszukiwanie w zupełnie nowy sposób bibliotek sekwencji kodujących  różne białka. </td>
+
    <td align="center" valign="bottom">
-
  <td><img src="spacer.gif" width="1" height="530" border="0" alt="" /></td>
+
<a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h4>Department of Bacterial Genetics </h4></a>
 +
 
 +
</td>
 +
<td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h4>Institute of Genetics and Biotechnology</h4></a></td>
   </tr>
   </tr>
</table>
</table>
-
<map name="m_igrm_home_r1_c1" id="m_igrm_home_r1_c1">
+
</td>
-
<area shape="rect" coords="404,217,546,260" href="igem_notebook.htm" alt="" />
+
<td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" />
-
<area shape="rect" coords="266,220,368,261" href="igem_project.htm" alt="" />
+
</td>
-
<area shape="rect" coords="144,219,232,259" href="igem_team.htm" alt="" />
+
</tr>
-
<area shape="rect" coords="10,219,104,257" alt="" />
+
</table>
-
</map>
+
 
-
</body>
+
</html>
</html>

Latest revision as of 21:27, 29 October 2008

Gallery Bricks Notebook Team Project Home

A novel system for generation and screening for new interactors targeted against any bait

  • maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter
  • interactor presentation on bacterial surface
  • selection of best bait interactor

Institutions whom we would like to thank for financial and technical support:

University of Warsaw

Faculty of Biology, University of Warsaw

The Universitatis Varsoviensis Foundation

Foundation for Polish Science

Warsaw University Foundation

Department of Molecular Biology, International Institute of Molecular and Cell Biology

Units belonging to the Faculty of Biology, University of Warsaw, who kindly provided access to laboratory space and equipment:

Department of Plant Molecular Biology

Department of Virology

Department of Bacterial Genetics

Institute of Genetics and Biotechnology

Retrieved from "http://2008.igem.org/Team:Warsaw"