Latest revision as of 21:27, 29 October 2008

A novel system for generation and screening for new interactors targeted against any bait

maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter
interactor presentation on bacterial surface
selection of best bait interactor

Institutions whom we would like to thank for financial and technical support:

University of Warsaw	Faculty of Biology, University of Warsaw
The Universitatis Varsoviensis Foundation	Foundation for Polish Science

Warsaw University Foundation	Department of Molecular Biology, International Institute of Molecular and Cell Biology
Units belonging to the Faculty of Biology, University of Warsaw, who kindly provided access to laboratory space and equipment:
Department of Plant Molecular Biology	Department of Virology
Department of Bacterial Genetics	Institute of Genetics and Biotechnology

@@ Line 2: / Line 2: @@
 <html>
-<p>Our team consists of 9 students of Faculty of Biology, University of
+<h1>A novel system for generation and screening for new interactors targeted against any bait</h1>
-Warsaw. This is the first time a group from Poland participates in the
+<p><ul>
-iGEM competition. Our plans were really amazing: we wanted to create
+<li>maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter</li><li>
-system of biological machines which would allow to investigate protein
+interactor presentation on bacterial surface</li><li>
-interactions and simultaneously change sequence of one of them in
+selection of best bait interactor</li>
-order to achieve the strongest possible interaction. To make it work
+</ul></p>
-we planned to express the tested protein in a bacterial strain in
+<br>
-which it would be mutated. Selection would occur outside outer
-bacterial membrane, where the protein would be attached. We planned
+<div align="center"><img src="https://static.igem.org/mediawiki/2008/6/6a/Krag_zycia2.gif" usemap="#Map"/>
-that such selection system would allow us to search for antibodies
+<map name="Map" id="Map">
-with new specificities or screen protein libraries. </p>
+  <area shape="poly" coords="596,397,458,396,454,334,598,331,593,337" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#selection" />
-<div align="center"><img src="https://static.igem.org/mediawiki/2008/6/6a/Krag_zycia2.gif"/>
+<area shape="poly" coords="346,434,234,422,154,661,337,666" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#mutagenesis" />
-<map name="main_scheme" id="Map">
+<area shape="poly" coords="210,265,278,225,323,227,372,250,376,254,582,53,534,5,395,1,6,1,30,157" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#hunter" />
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+<area shape="poly" coords="185,349,211,388,23,470,9,385" href="https://2008.igem.org/Wiki/Team:Warsaw/igem_project.htm#selection" />
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+</map>
 </div>
 </td>
@@ Line 23: / Line 23: @@
    </tr>
 <tr><td>
-<h2>Institutions whom we would like to thank for financial and technical support:</h2>
+<h3>Institutions whom we would like to thank for financial and technical support:</h3>
 <table width="100%" border="0" cellspacing="10" cellpadding="0" align="center">
    <tr>
      <td align="center" valign="bottom"><a href="http://www.uw.edu.pl/en/"><img src="https://static.igem.org/mediawiki/2008/6/67/Uw.gif" width="100" height="99" border="0" /></a>
-     <a href="http://www.uw.edu.pl/en/"><h3>University of Warsaw</h3> </a></td>
+     <a href="http://www.uw.edu.pl/en/"><h4>University of Warsaw</h4> </a></td>
      <td align="center" valign="bottom">
 <a href="http://www.biol.uw.edu.pl/_2008eng/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="57" height="75" border="0" /></a>
-     <a href="http://www.biol.uw.edu.pl/_2008eng/"><h3>Faculty of Biology, University of Warsaw </h3></a>
+     <a href="http://www.biol.uw.edu.pl/_2008eng/"><h4>Faculty of Biology, University of Warsaw </h4></a>
 </td>
    </tr>
    <tr>
      <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a>
-     <a href="http://www.fundacjauv.org.pl/"><h3>The Universitatis Varsoviensis Fund </h3></a></td>
+     <a href="http://www.fundacjauv.org.pl/"><h4>The Universitatis Varsoviensis Foundation </h4></a></td>
      <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="221" height="75" border="0" /></a>
-     <a href="http://www.fnp.org.pl/ang/index.html"><h3>Foundation for Polish Science </h3></a></td>
+     <a href="http://www.fnp.org.pl/ang/index.html"><h4>Foundation for Polish Science </h4></a></td>
    </tr>
    <tr>
@@ Line 45: / Line 45: @@
    <tr>
      <td align="center" valign="bottom">
-<a href="http://www.fuw.pl"><H3>Warsaw University Foundation</h3></a>
+<a href="http://www.fuw.pl"><h4>Warsaw University Foundation</h4></a>
 </td>
      <td align="center" valign="bottom">
-<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h3>Department of Molecular Biology, IIMCB</h3></a>
+<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h4>Department of Molecular Biology, International Institute of Molecular and Cell Biology</h4></a>
 </td>
    </tr>
@@ Line 55: / Line 55: @@
 <tr>
 <td colspan="2" align="center" valign="bottom">
-<p>Departments belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
+<p>Units belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
 </td>
    <tr>
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h3>Department of Plant Molecular Biology </h3></a></td>
+     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h4>Department of Plant Molecular Biology </h4></a></td>
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h3>Department of Virology</h3> </a> </td>
+     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h4>Department of Virology</h4> </a> </td>
    </tr>
    <tr>
       <td align="center" valign="bottom">
-<a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h3>Department of Bacterial Genetics </h3></a>
+<a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h4>Department of Bacterial Genetics </h4></a>
 </td>
-<td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h3>Institute of Genetics and Biotechnology</h3></a></td>
+<td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h4>Institute of Genetics and Biotechnology</h4></a></td>
    </tr>
 </table>