Latest revision as of 21:27, 29 October 2008

A novel system for generation and screening for new interactors targeted against any bait

maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter
interactor presentation on bacterial surface
selection of best bait interactor

Institutions whom we would like to thank for financial and technical support:

University of Warsaw	Faculty of Biology, University of Warsaw
The Universitatis Varsoviensis Foundation	Foundation for Polish Science

Warsaw University Foundation	Department of Molecular Biology, International Institute of Molecular and Cell Biology
Units belonging to the Faculty of Biology, University of Warsaw, who kindly provided access to laboratory space and equipment:
Department of Plant Molecular Biology	Department of Virology
Department of Bacterial Genetics	Institute of Genetics and Biotechnology

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+<h1>A novel system for generation and screening for new interactors targeted against any bait</h1>
+<p><ul>
+<li>maximization of interaction between given bait and randomly generated protein by mutating sequence of the latter</li><li>
+interactor presentation on bacterial surface</li><li>
+selection of best bait interactor</li>
+</ul></p>
 <br>
-<p>We have developed a system allowing to search for antibodies with new specificities or screen protein libraries in order to generate interactors for a given bait. Our system changes a protein sequence to maximize its interaction with a given partner. Proteins with modified sequences are then directed to the bacterial outer membrane, where the best interactors are selected. Protein presented on the cell surface is fused with part of &#946;-lactamase protein, while its bait is fused with the complementing part of the enzyme and added to medium. The stronger the interaction between proteins of interest, the more efficient the binding of the two halves of &#946;-lactamase, leading to resistance to ampicillin and survival. Cells expressing less interacting variants die as they don't achieve sufficient complementation of the reporter enzyme. This allows us to select strains producing interactors for any given bait. </p>
-<br>
-<br>
-Pisze tu bo boje się żejutro zapomnię - selekcja powinna być po mutagenezie, a nie przed, albo jeszcze lepiej po mutagenezie druga selekcja bo teraz to trochę wygląda jakbyśmy odrazu po mutagenezie otrzymywali białko oddziałujące
-<br><br>
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    </tr>
 <tr><td>
-<h2>Institutions whom we would like to thank for financial and technical support:</h2>
+<h3>Institutions whom we would like to thank for financial and technical support:</h3>
 <table width="100%" border="0" cellspacing="10" cellpadding="0" align="center">
    <tr>
      <td align="center" valign="bottom"><a href="http://www.uw.edu.pl/en/"><img src="https://static.igem.org/mediawiki/2008/6/67/Uw.gif" width="100" height="99" border="0" /></a>
-     <a href="http://www.uw.edu.pl/en/"><h3>University of Warsaw</h3> </a></td>
+     <a href="http://www.uw.edu.pl/en/"><h4>University of Warsaw</h4> </a></td>
      <td align="center" valign="bottom">
 <a href="http://www.biol.uw.edu.pl/_2008eng/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="57" height="75" border="0" /></a>
-     <a href="http://www.biol.uw.edu.pl/_2008eng/"><h3>Faculty of Biology, University of Warsaw </h3></a>
+     <a href="http://www.biol.uw.edu.pl/_2008eng/"><h4>Faculty of Biology, University of Warsaw </h4></a>
 </td>
    </tr>
    <tr>
      <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a>
-     <a href="http://www.fundacjauv.org.pl/"><h3>The Universitatis Varsoviensis Fund </h3></a></td>
+     <a href="http://www.fundacjauv.org.pl/"><h4>The Universitatis Varsoviensis Foundation </h4></a></td>
      <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="221" height="75" border="0" /></a>
-     <a href="http://www.fnp.org.pl/ang/index.html"><h3>Foundation for Polish Science </h3></a></td>
+     <a href="http://www.fnp.org.pl/ang/index.html"><h4>Foundation for Polish Science </h4></a></td>
    </tr>
    <tr>
@@ Line 41: / Line 45: @@
    <tr>
      <td align="center" valign="bottom">
-<a href="http://www.fuw.pl"><H3>Warsaw University Foundation</h3></a>
+<a href="http://www.fuw.pl"><h4>Warsaw University Foundation</h4></a>
 </td>
      <td align="center" valign="bottom">
-<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h3>Department of Molecular Biology, IIMCB</h3></a>
+<a href="http://www.iimcb.gov.pl/zylicz_lab.php"><h4>Department of Molecular Biology, International Institute of Molecular and Cell Biology</h4></a>
 </td>
    </tr>
@@ Line 51: / Line 55: @@
 <tr>
 <td colspan="2" align="center" valign="bottom">
-<p>Departments belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
+<p>Units belonging to the <a href="http://www.biol.uw.edu.pl/_2008eng/">Faculty of Biology, University of Warsaw</a>, who kindly provided access to laboratory space and equipment:</p>
 </td>
    <tr>
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h3>Department of Plant Molecular Biology </h3></a></td>
+     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&amp;b_2501=5"><h4>Department of Plant Molecular Biology </h4></a></td>
-     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h3>Department of Virology</h3> </a> </td>
+     <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&amp;b_2501=5"><h4>Department of Virology</h4> </a> </td>
    </tr>
    <tr>
       <td align="center" valign="bottom">
-<a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h3>Department of Bacterial Genetics </h3></a>
+<a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&amp;b_2501=5"><h4>Department of Bacterial Genetics </h4></a>
 </td>
-<td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h3>Institute of Genetics and Biotechnology</h3></a></td>
+<td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&amp;b_2501=5"><h4>Institute of Genetics and Biotechnology</h4></a></td>
    </tr>
 </table>