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Edinburgh/22 August 2008
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* Sequencing results received for M150 to M152: | * Sequencing results received for M150 to M152: | ||
** M150 is pSB1A2+''cex'' (although sequence quality collapsed after ~500bp). This has been resequenced with the pSB1A2insR2 primer to check the suffix. | ** M150 is pSB1A2+''cex'' (although sequence quality collapsed after ~500bp). This has been resequenced with the pSB1A2insR2 primer to check the suffix. | ||
| - | ** M151 looks to be a contaminant (possibly streptomyces) rather than cex. | + | ** M151 looks to be a contaminant (possibly streptomyces) rather than ''cex''. |
| - | ** M152 is pSB1A2+dxs+crtE. (AH) | + | ** M152 is pSB1A2+''dxs+crtE''. (AH) |
* Transformation results from yesterday: | * Transformation results from yesterday: | ||
| - | ** SOB2-glgC: Plates 145, 146 and 152 had no colonies at all. Other plates had very few white colonies and no blue colonies. | + | ** SOB2-''glgC'': Plates 145, 146 and 152 had no colonies at all. Other plates had very few white colonies and no blue colonies. |
| - | ** | + | ** ''lacZ''-P''cstA'': Plate 147 - 3~4 white, 0 blue; Plate 154 - 67 white, 5 blue. (AM) |
| - | * Hence, subbed colonies from plates on which there was growth to '''Plates 158~163''' (see list of plates for details). White and blue colonies from plates 147/154 ( | + | * Hence, subbed colonies from plates on which there was growth to '''Plates 158~163''' (see list of plates for details). White and blue colonies from plates 147/154 (''lacZ''-P''cstA'') were plated separately onto '''Plates 162 and 163''' respectively. (AM) |
Latest revision as of 21:59, 29 October 2008
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Week 10
Friday 22 August 2008
- Sequencing results received for M150 to M152:
- M150 is pSB1A2+cex (although sequence quality collapsed after ~500bp). This has been resequenced with the pSB1A2insR2 primer to check the suffix.
- M151 looks to be a contaminant (possibly streptomyces) rather than cex.
- M152 is pSB1A2+dxs+crtE. (AH)
- Transformation results from yesterday:
- SOB2-glgC: Plates 145, 146 and 152 had no colonies at all. Other plates had very few white colonies and no blue colonies.
- lacZ-PcstA: Plate 147 - 3~4 white, 0 blue; Plate 154 - 67 white, 5 blue. (AM)
- Hence, subbed colonies from plates on which there was growth to Plates 158~163 (see list of plates for details). White and blue colonies from plates 147/154 (lacZ-PcstA) were plated separately onto Plates 162 and 163 respectively. (AM)
