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Team:Chiba/Calendar-Home/6 September 2008
From 2008.igem.org
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===Team:Communication=== | ===Team:Communication=== | ||
'''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' | '''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' | ||
| - | ::PCR of BBa_I9030 and two control, | + | ::PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] and two control,[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)). |
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| Line 56: | Line 56: | ||
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | ||
| - | + | {|align="justify" | |
| + | |[[Image:Chiba-0906.JPG|300px]] | ||
| + | | | ||
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
| Line 72: | Line 74: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>6μl</td></tr> |
</table> | </table> | ||
:From left;<br> | :From left;<br> | ||
| - | :1 | + | :1 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] without LVA -> OK |
| - | :2 | + | :2 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] Positive Control -> OK |
| - | :3 | + | :3 [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] Positive control -> OK |
| + | |} | ||
'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | '''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | ||
| - | Sample DNA : | + | {|align="justify" |
| + | |[[Image:Chiba-0906-2.JPG|300px]] | ||
| + | | | ||
| + | Sample DNA : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | ||
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
| Line 96: | Line 102: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>6μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| - | + | :R0010 --> 100μL | |
| + | |} | ||
| Line 105: | Line 112: | ||
'''[[Team:Chiba/protocol/digestion|Digestion Test]]''' | '''[[Team:Chiba/protocol/digestion|Digestion Test]]''' | ||
:Sample DNA: | :Sample DNA: | ||
| - | 1: | + | 1:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010],2:[http://partsregistry.org/Part:BBa_S03154 BBa_S03154],3:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500],4:[http://partsregistry.org/Part:BBa_C0161 BBa_C0161],5:[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] |
| + | |||
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| Line 115: | Line 123: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>PstI</td> |
<td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td> | <td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>SpeI</td> |
<td>0.5</td><td>-</td><td>1</td><td>-</td><td>-</td> | <td>0.5</td><td>-</td><td>1</td><td>-</td><td>-</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>XbaI</td> |
<td>-</td><td>0.5</td><td>-</td><td>0.5</td><td>0.5</td> | <td>-</td><td>0.5</td><td>-</td><td>0.5</td><td>0.5</td> | ||
</tr> | </tr> | ||
| Line 148: | Line 156: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>20μl</td><td>60μl</td><td>30μl</td><td>60μl</td><td>60μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 22:19, 29 October 2008
5 September 2008 <|> 7 September 2008
Contents |
Meeting with UmeG
the ppt (in Japanese) for our weekly meeting
Laboratory work
Team:Input
no work
Team:Communication
- PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] and two control,[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template 1 dNTP mix 5 Foward Primer 5 Reverse Primer 5 DNA polymerase 1 Thermopol Buffer 5 dH2O 33 TOTAL 50μL
- 95°C,5min -> ( 95°C,1min -> 54°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C store
- --->Gel Check
Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6μl - From left;
- 1 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] without LVA -> OK
- 2 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] Positive Control -> OK
- 3 [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] Positive control -> OK
- From left;
Sample DNA : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6μl - R0010 --> 100μL
- Sample DNA:
1:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010],2:[http://partsregistry.org/Part:BBa_S03154 BBa_S03154],3:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500],4:[http://partsregistry.org/Part:BBa_C0161 BBa_C0161],5:[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]
Sample No. 1 2 3 4 5 Sample DNA 1 1 1 1 1 PstI 0.5 0.5 0.5 0.5 0.5 SpeI 0.5 - 1 - - XbaI - 0.5 - 0.5 0.5 Buffer 2 2 - 3 - - Buffer 3 - 6 - 6 6 (10×)BSA 2 0.6 3 - - (100×)BSA - - - 0.6 0.6 dH2O 3 6.4 7 1.4 1.4 TOTAL 20μl 60μl 30μl 60μl 60μl Team:Output
no work
