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Team:Chiba/Calendar-Home/14 September 2008
From 2008.igem.org
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| - | <td> | + | <td>EcoRI(μL)</td> |
<td>0.075</td> | <td>0.075</td> | ||
</tr> | </tr> | ||
Revision as of 22:24, 29 October 2008
13 September 2008 <|> 15 September 2008
Laboratory work
Team:Communication
- (13/9)--->Mini prep
- Transformation
- Competent cells : XL10Gold
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
- --->(15/9)
Team:Output
- [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
- [http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
- [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
- [http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
- [http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
- [http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
- BBa_I716542
- [http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
- [http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
- [http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
- [http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
- [http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
- [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
- [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
- [http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
- [http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
- [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](×2)
- [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
| Sample No. | each |
| DNA(digested sample)(μL) | 3 |
| Buffer2(x10)(μL) | 1 |
| dH2O(μL) | 6 |
| EcoRI(μL) | 0.075 |
| TOTAL(μL) | 10 |
--> left at 37°C for 1 hour
| Sample No. | each |
| DNA(digestion sample)(μL) | 10 |
| Loading Dye(μL) | 2 |
| TOTAL(μL) | 12 |
