Team:Chiba/Calendar-Home/17 September 2008

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(Difference between revisions)

Latest revision as of 22:35, 29 October 2008

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16 September 2008 <|> 18 September 2008

Laboratory work

Team:Communication

time-delay test
- transformants
  - [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
  - [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
  - [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
  - [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
- E.coli strain
  Senders:JW1908
  Receiver:JW1908
- reaction temparature
  37°C and 30°C

time-delay test
- transformants
  - [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
  - [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
  - [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
- E.coli strain
  Senders:XL10GOLD
  Receiver:JW1908
- reaction temparature
  37°C and 30°C

Team:Output

Transformed

[http://partsregistry.org/Part:BBa_I13500 BBa_I13500]
[http://partsregistry.org/Part:BBa_J37019 BBa_J37019]-->no colony

miniprepped

[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
[http://partsregistry.org/Part:BBa_J37032 BBa_J37032]

Digestion

[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 BBa_K084004]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 BBa_K084005]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 BBa_K084006]

Sample No.	each
DNA template(μL)	25
EcoRI(μL)	1
PstI(μL)	1
BSA(×10)(μL)	4
EcoRI Buffer(μL)	4
dH₂O(μL)	5
TOTAL(μL)	40

-->Left at 37 °C for 1 hour.

-->Next day

@@ Line 7: / Line 7: @@
 ===Team:Communication===
 *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-*transformants
+**transformants
-*[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
+***[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
-*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
+***[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
-*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
+***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
-*E.coli strain<br>Senders:JW1908<br>Receiver:JW1908<br>
+***[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
-*reaction temparature<br>37°C and 30°C
+**E.coli strain<br>Senders:JW1908<br>Receiver:JW1908<br>
+**reaction temparature<br>37&deg;C and 30&deg;C
 *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-*transformants
+**transformants
-*[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
+***[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
-*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
+***[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
-*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
+***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
-*E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
+**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
-*reaction temparature<br>37°C and 30°C
+**reaction temparature<br>37&deg;C and 30&deg;C
 ===Team:Output===
 [[Team:Chiba/protocol/transformation|Transformed]]
-*[http://partsregistry.org/Part:BBa_I13500 BBa_I13500](RBS+GFP)
+*[http://partsregistry.org/Part:BBa_I13500 BBa_I13500]
-*[http://partsregistry.org/Part:BBa_J37019 BBa_J37019](Luxpr+LuxR)-->no colony
+*[http://partsregistry.org/Part:BBa_J37019 BBa_J37019]-->no colony
 [[Team:Chiba/protocol/DNA_Purification/sigma|miniprepped]]
-*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
+*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
-*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020](Luxpr+LuxR+LuxR+GFP)
+*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
-*[http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)
+*[http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
 [[Team:Chiba/protocol/digestion|Digestion]]
-*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](ptetR+RBS)
+*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
-*[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
+*[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
-*Ligation product(ptet+LasR)
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 BBa_K084004]
-*Ligation product(ptet+RhlR)
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 BBa_K084005]
-*Ligation product(ptet+cinR)
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 BBa_K084006]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
@@ Line 47: / Line 49: @@
 </tr>
 <tr>
-<td>DNA template(μL)</td>
+<td>DNA template(&mu;L)</td>
 <td>25</td>
 </tr>
 <tr>
-<td>EcoRⅠ(μL)</td>
+<td>EcoRI(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>PstⅠ(μL)</td>
+<td>PstI(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>BSA(x10)(μL)</td>
+<td>BSA(&times;10)(&mu;L)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>EcoRⅠ Buffer(μL)</td>
+<td>EcoRI Buffer(&mu;L)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O(μL)</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>TOTAL(μL)</td>
+<td>TOTAL(&mu;L)</td>
 <td>40</td>
 </tr>
 </table>
--->Left at37 degrees for 1 hour.
+-->Left at 37 &deg;C for 1 hour.
 -->Next day