Team:Chiba/Calendar-Home/20 September 2008
From 2008.igem.org
(Difference between revisions)
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<tr> | <tr> | ||
<td width="257">Sample Name</td> | <td width="257">Sample Name</td> | ||
- | <td>Lagation( | + | <td>Lagation([http://partsregistry.org/Part:BBa_J04500 BBa_J04500]+[http://partsregistry.org/Part:BBa_C0161 BBa_C0161])</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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--->(22/9)'''phenotype check | --->(22/9)'''phenotype check | ||
- | |||
===Team:Output=== | ===Team:Output=== | ||
no work | no work |
Revision as of 22:55, 29 October 2008
19 September 2008 <|> 21 September 2008
Laboratory work
Team:Communication
--->(19/9)Mini prep
--->Gel Check
Sample Name | Lagation([http://partsregistry.org/Part:BBa_J04500 BBa_J04500]+[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]) |
Sample DNA(μL) | 1 |
Loading Dye(μL) | 1 |
H2O(μL) | 4 |
TOTAL(μL) | 6 |
- From left;
- -> OK
- -> OK
- -> OK
- -> OK
- --->Digestion Test
Sample No. | 1-4 | 5-8 |
Sample DNA(μL) | 3 | 3 |
XbaI | 0.25? | 0.25 |
SpeI | 0.5 | 0.5 |
Buffer 2(μL) | 2 | 2 |
BSA(μL) | 2 | 2 |
dH2O(μL) | ? | 12.5 |
TOTAL(μL) | 20 | 20 |
- --->Gel Check
Sample No. 1-4 5~8 Sample DNA(μL) 20 20 Loading Dye(μL) 4 4 TOTAL(μL) 24 24 - From left;
- -> OK
- -> OK
- -> OK
- -> OK
- -> OK
- -> OK
- -> OK
- -> OK
--->(?/9)Transformation
--->(22/9)phenotype check
Team:Output
no work
- From left;