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Team:The University of Alberta/27 June 2008
From 2008.igem.org
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(New page: ==Today== *The O/Ns that were set up last night turned out well. Did mini-preps on them today. *The transformations done yesterday turned out perfectly. Now [[:Image:june27_transformants|...) |
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*Gel purified ligations of all the parts we were missing in J61003 (21, 22, 25, 35, 99); also purifying J61003 because we have run out! | *Gel purified ligations of all the parts we were missing in J61003 (21, 22, 25, 35, 99); also purifying J61003 because we have run out! | ||
*More transformations: this time on the parts listed above in J61003 | *More transformations: this time on the parts listed above in J61003 | ||
| + | *Made SDD-PAGE gels to run the purified His-tagged proteins on. | ||
Revision as of 20:02, 27 June 2008
Today
- The O/Ns that were set up last night turned out well. Did mini-preps on them today.
- The transformations done yesterday turned out perfectly. Now this is what transformations should look like!
- Running colony PCR as a test for contamination to explain the strange results we got yesterday
- Gel purified ligations of all the parts we were missing in J61003 (21, 22, 25, 35, 99); also purifying J61003 because we have run out!
- More transformations: this time on the parts listed above in J61003
- Made SDD-PAGE gels to run the purified His-tagged proteins on.
