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Team:Chiba/Calendar-Home/14 September 2008
From 2008.igem.org
(Difference between revisions)
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==Laboratory work== | ==Laboratory work== | ||
===Team:Communication=== | ===Team:Communication=== | ||
| - | + | ||
| + | (13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' | ||
::*[http://partsregistry.org/Part:BBa_I7106 BBa_I7106] | ::*[http://partsregistry.org/Part:BBa_I7106 BBa_I7106] | ||
::*[http://partsregistry.org/Part:BBa_I23019 BBa_I23019] | ::*[http://partsregistry.org/Part:BBa_I23019 BBa_I23019] | ||
| + | (13/9)--->[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]] | ||
| + | ::*[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] | ||
| + | ::*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | ||
| + | :--->(19/9) | ||
| - | + | '''[[Team:Chiba/protocol/transformation|Transformation]]''' | |
:Competent cells : XL10Gold | :Competent cells : XL10Gold | ||
::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007) | ::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007) | ||
| Line 17: | Line 22: | ||
::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA) | ::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA) | ||
| - | :--->(15/9) | + | :--->(15/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' |
===Team:Output=== | ===Team:Output=== | ||
Revision as of 23:55, 29 October 2008
13 September 2008 <|> 15 September 2008
Laboratory work
Team:Communication
(13/9)--->Mini prep
- [http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
- [http://partsregistry.org/Part:BBa_I23019 BBa_I23019]
(13/9)--->[[1]]
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
- --->(19/9)
- Competent cells : XL10Gold
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
- --->(15/9)Gel Check
Team:Output
- [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
- [http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
- [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
- [http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
- [http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
- [http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
- [http://partsregistry.org/Part:BBa_I716452 BBa_I716452]
- [http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
- [http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
- [http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
- [http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
- [http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
- [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
- [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
- [http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
- [http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
- [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](×2)
- [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
| Sample No. | each |
| DNA(digested sample)(μL) | 3 |
| Buffer2(x10)(μL) | 1 |
| dH2O(μL) | 6 |
| EcoRI(μL) | 0.075 |
| TOTAL(μL) | 10 |
--> left at 37°C for 1 hour
| Sample No. | each |
| DNA(digestion sample)(μL) | 10 |
| Loading Dye(μL) | 2 |
| TOTAL(μL) | 12 |
