Team:Chiba/Calendar-Home/14 September 2008

From 2008.igem.org

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(New page: >Home | Notebook 13 September 2008 <|[[Team:Chiba/Calendar-Home/15 September 2008|> 15 September 2008...)
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==Laboratory work==
==Laboratory work==
===Team:Communication===
===Team:Communication===
 +
 +
(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
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::*[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
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::*[http://partsregistry.org/Part:BBa_I23019 BBa_I23019]
 +
 +
(13/9)--->[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
 +
::*[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
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::*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
 +
 +
:--->(19/9)
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 +
'''[[Team:Chiba/protocol/transformation|Transformation]]'''
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:Competent cells : XL10Gold
 +
::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
 +
::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
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::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
 +
 +
:--->(19/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
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 +
 +
'''[[Team:Chiba/protocol/Time Delay Test|Time Delay Test]]'''
 +
 +
 +
===Team:Output===
===Team:Output===
 +
 +
[[Team:Chiba/jk/γ/Trl|Time Responce Liquid]]
 +
 +
[[Team:Chiba/protocol/digestion|Digestion test]]
 +
 +
*[http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
 +
*[http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
 +
*[http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
 +
*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
 +
*[http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
 +
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
 +
*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
 +
*[http://partsregistry.org/Part:BBa_I716452 BBa_I716452]
 +
*[http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
 +
*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
 +
*[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
 +
*[http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
 +
*[http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
 +
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
 +
*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
 +
*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
 +
*[http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
 +
*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](&times;2)
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*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No.</td>
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<td>each</td>
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</tr>
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<tr>
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<td>DNA(digested sample)(&mu;L)</td>
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<td>3</td>
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</tr>
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<tr>
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<td>Buffer2(x10)(&mu;L)</td>
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<td>1</td>
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</tr>
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<tr>
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<td>dH<sub>2</sub>O(&mu;L)</td>
 +
<td>6</td>
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</tr>
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<tr>
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<td>EcoRI(&mu;L)</td>
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<td>0.075</td>
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</tr>
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<tr>
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<td>TOTAL(&mu;L)</td>
 +
<td>10</td>
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</tr>
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</table>
 +
 +
--> left at 37&deg;C for 1 hour
 +
 +
[[Team:Chiba/protocol/gelcheck|Gel check]]
 +
 +
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No.</td>
 +
<td>each</td>
 +
</tr>
 +
<tr>
 +
<td>DNA(digestion sample)(&mu;L)</td>
 +
<td>10</td>
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</tr>
 +
<tr>
 +
<td>Loading Dye(&mu;L)</td>
 +
<td>2</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL(&mu;L)</td>
 +
<td>12</td>
 +
</tr>
 +
</table>

Latest revision as of 23:59, 29 October 2008

>Home | Notebook

13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep

  • [http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
  • [http://partsregistry.org/Part:BBa_I23019 BBa_I23019]

(13/9)--->[[1]]

  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
--->(19/9)

Transformation

Competent cells : XL10Gold
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
--->(19/9)Gel Check


Time Delay Test



Team:Output

Time Responce Liquid

Digestion test

  • [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
  • [http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
  • [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
  • [http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
  • [http://partsregistry.org/Part:BBa_I716452 BBa_I716452]
  • [http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
  • [http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
  • [http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
  • [http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](×2)
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
Sample No. each
DNA(digested sample)(μL) 3
Buffer2(x10)(μL) 1
dH2O(μL) 6
EcoRI(μL) 0.075
TOTAL(μL) 10

--> left at 37°C for 1 hour

Gel check

Sample No. each
DNA(digestion sample)(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/14_September_2008"