Team:Chiba/Calendar-Home/16 September 2008

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Latest revision as of 00:00, 30 October 2008

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15 September 2008 <|> 17 September 2008

Laboratory work

Team:Communication

time-delay test with (15/9) cultures.

reaction temparature was 37°C and 30°C.

result

Created Glycerol Stocks from (15/9) cultures.

Team:Output

Digestion test

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 BBa_K084004](×2)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 BBa_K084005](×5)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 BBa_K084006](×2)
[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]
[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]
[http://partsregistry.org/Part:BBa_S03160 BBa_S03160]

Sample No.	each
DNA(μL)	3
EcoRI(μL)	0.083
PstI(μL)	0.083
BSA(×10)(μL)	1
Buffer3(×10)(μL)	1
dH₂O(μL)	5
TOTAL(μL)	10

-->37°C 1 hour

Gel check

Sample No.	each
DNA(μL)	10
Loading Dye(μL)	2
TOTAL(μL)	12

Transformation

[http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
[http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
[http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
[http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

PCR

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]

Sample No.	[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
DNA(μL)	1
Venus Fwd(μL)	5
Rev(VR)(μL)	5
Thermo pol Buffer(μL)	10
dNTP mix(μL)	10
Vent(μL)	1
dH₂O(μL)	68
TOTAL(μL)	100

@@ Line 6: / Line 6: @@
 ===Team:Communication===
-(15/9)->
+[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] with (15/9) cultures.
-[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
+*reaction temparature was 37&deg;C and 30&deg;C.
-*[[Team:Chiba/protocol/transformation|Transformed]] these plasmids　into E.coli strain JW1908.(also transformed [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] into E.Coli strain JW1908.)
-**[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
+*result
-**[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
-**[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
+*Created Glycerol Stocks from (15/9) cultures.
-**[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
-*conditions
-#temparature<br>37℃ and 30℃
 ===Team:Output===
 <br>Digestion test
-*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(x2)
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 BBa_K084004](&times;2)
-*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(x5)
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 BBa_K084005](&times;5)
-*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(x2)
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 BBa_K084006](&times;2)
-*[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
+*[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]
-*[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
+*[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]
-*[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)
+*[http://partsregistry.org/Part:BBa_S03160 BBa_S03160]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
@@ Line 33: / Line 32: @@
 </tr>
 <tr>
-<td>DNA(μL)</td>
+<td>DNA(&mu;L)</td>
 <td>3</td>
 </tr>
 <tr>
-<td>EcoRⅠ(μL)</td>
+<td>EcoRI(&mu;L)</td>
 <td>0.083</td>
 </tr>
 <tr>
-<td>PstⅠ(μL)</td>
+<td>PstI(&mu;L)</td>
 <td>0.083</td>
 </tr>
 <tr>
-<td>BSA(x10)(μL)</td>
+<td>BSA(&times;10)(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>Buffer3(x10)(μL)</td>
+<td>Buffer3(&times;10)(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O(μL)</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>TOTAL(μL)</td>
+<td>TOTAL(&mu;L)</td>
 <td>10</td>
 </tr>
 </table>
--->37℃ 1hour
+-->37&deg;C 1 hour
 [[Team:Chiba/protocol/gelcheck|Gel check]]
@@ Line 72: / Line 71: @@
 </tr>
 <tr>
-<td>DNA(μL)</td>
+<td>DNA(&mu;L)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>Loading Dye(μL)</td>
+<td>Loading Dye(&mu;L)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>TOTAL(μL)</td>
+<td>TOTAL(&mu;L)</td>
 <td>12</td>
 </tr>
@@ Line 92: / Line 91: @@
 [[Team:Chiba/protocol/PCR|PCR]]
-*Venus YFP(ptet+LuxR+YFP)
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No.</td>
-<td>YFP</td>
+<td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]</td>
 </tr>
 <tr>
-<td>DNA(μL)</td>
+<td>DNA(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>Venus Fwd(μL)</td>
+<td>Venus Fwd(&mu;L)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>Rev(VR)(μL)</td>
+<td>Rev(VR)(&mu;L)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>Thermo pol Buffer(μL)</td>
+<td>Thermo pol Buffer(&mu;L)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>dNTP mix(μL)</td>
+<td>dNTP mix(&mu;L)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>Vent(μL)</td>
+<td>Vent(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O(μL)</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>68</td>
 </tr>
 <tr>
-<td>TOTAL(μL)</td>
+<td>TOTAL(&mu;L)</td>
 <td>100</td>
 </tr>
 </table>