Team:Chiba/Calendar-Home/20 September 2008

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(Difference between revisions)
(→Team:Communication)
(→Team:Communication)
Line 108: Line 108:
::#　-> OK
::#　-> OK
::#　-> OK
::#　-> OK
 +
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--->(?/9)'''[[Team:Chiba/protocol/transformation|Transformation]]'''
--->(?/9)'''[[Team:Chiba/protocol/transformation|Transformation]]'''

Revision as of 00:24, 30 October 2008

>Home | Notebook

19 September 2008 <|> 21 September 2008

Laboratory work

Team:Communication

--->(19/9)Mini prep

--->Gel Check

Chiba-0920.JPG
Sample Name Lagation([http://partsregistry.org/Part:BBa_J04500 BBa_J04500]+[http://partsregistry.org/Part:BBa_C0161 BBa_C0161])
Sample DNA(μL) 1
Loading Dye(μL) 1
H2O(μL) 4
TOTAL(μL) 6
From left;
  1. 　-> OK
  2. 　-> OK
  3. 　-> OK
  4. 　-> OK
--->Digestion Test
Sample No. 1-45-8
Sample DNA(μL) 33
XbaI 0.25?0.25
SpeI 0.50.5
Buffer 2(μL) 22
BSA(μL) 22
dH2O(μL) ?12.5
TOTAL(μL) 2020


--->Gel Check
Chiba-0920-2.JPG :
Sample No. 1-45~8
Sample DNA(μL) 2020
Loading Dye(μL) 44
TOTAL(μL) 2424
From left;
  1. 　-> OK
  2. 　-> OK
  3. 　-> OK
  4. 　-> OK
  5. 　-> OK
  6. 　-> OK
  7. 　-> OK
  8. 　-> OK

--->(?/9)Transformation

--->(22/9)phenotype check

Team:Output

no work

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