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Team:Hawaii/PCC6803 cell density
From 2008.igem.org
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'''Cell density for OD750=1.159 was approximately 6.65 x 10<sup>7</sup>.''' | '''Cell density for OD750=1.159 was approximately 6.65 x 10<sup>7</sup>.''' | ||
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| + | == Discussion== | ||
| + | [[Image:Growth_curve.JPG|right|Growth curve (semi-log plot) of ''Synechocystis'' sp. PCC6803 cells inoculated in BG-11 medium (open circles), marine medium (closed circles), and twice normal marine medium (closed boxes).]] | ||
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| + | Reference:<br> | ||
| + | Richardson, D.L. et. Al., “Synechocystis PCC6803: a euryhaline cyanobacterium,” FEMS Microbiology Letters 18 (1983): 99-102. | ||
Latest revision as of 21:53, 27 June 2008
Contents |
Abstract
We would like to correlate OD750 with specific cell densities (# cells/ml)
Protocol
- Plated 200 μl of 1, 10-1, 10-2, 10-5, 10-7, and 10-8 dilutions of PCC6803 on BG-11.
- Incubated at 37C with illumination in the SC lab for 12 days.
Results
| Concentration | OD 750 = 1.159 | OD 750 = 0.071 |
|---|---|---|
| 1 | lawn | lawn |
| 10-1 | lawn | lawn |
| 10-2 | lawn | lawn |
| 10-5 | 133 | 48 |
| 10-7 | 0 | 1 |
| 10-8 | 0 | 0 |
Cell density was determined using the 10-5 colony count as it was the only statistically significant number (30<x<300).
Cell density for OD750=0.071 was approximately 2.40 x 107.
Cell density for OD750=1.159 was approximately 6.65 x 107.
Discussion
Reference:
Richardson, D.L. et. Al., “Synechocystis PCC6803: a euryhaline cyanobacterium,” FEMS Microbiology Letters 18 (1983): 99-102.
