Team:Chiba/Calendar-Home/11 October 2008

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(New page: 朝senderをwashして菌液10mlとLB-agar10mlを混ぜて固まらないうちにプレートに流し込む。 receiverをニトロセルロースで写し取り、senderのプレー...)
 
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朝senderをwashして菌液10mlとLB-agar10mlを混ぜて固まらないうちにプレートに流し込む。
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>[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
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[[Team:Chiba/Calendar-Home/10 October 2008|10 October 2008 <]]|[[Team:Chiba/Calendar-Home/12 October 2008|> 12 October 2008]]
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receiverをニトロセルロースで写し取り、senderのプレートにはる。そこをスタートとして37℃の培養機にいれ、30分ごとにUVを当ててGFPが出ているかどうかを確認する。
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==Laboratory work==
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===Team:Demo-Rs===
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~10 October
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#Sender Wash
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##Centrifuged 2 tubes containing([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))at 20&deg;C,3600rpm for 6min and discarded supernatant.
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##Added 10mL LB-Amp to each tube.
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##Repeated wash twice.
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#Creating bacterial plates
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##LB-Amp pre-cultured Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908)) tube(10mL) was mixed with LB-Amp-agar(50&deg;C)(10ml)to produce sender containing bacterialplate.
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#Lifted with nitrocellulose
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##Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908))containing bacterial plate and Sender-absent negative control plate (t=0).
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#Method to detect fluorescence
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##Plates cultured at 37&deg;C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.

Latest revision as of 00:38, 30 October 2008

>Home | Notebook

10 October 2008 <|> 12 October 2008

Laboratory work

Team:Demo-Rs

~10 October

  1. Sender Wash
    1. Centrifuged 2 tubes containing([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))at 20°C,3600rpm for 6min and discarded supernatant.
    2. Added 10mL LB-Amp to each tube.
    3. Repeated wash twice.
  2. Creating bacterial plates
    1. LB-Amp pre-cultured Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908)) tube(10mL) was mixed with LB-Amp-agar(50°C)(10ml)to produce sender containing bacterialplate.
  3. Lifted with nitrocellulose
    1. Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908))containing bacterial plate and Sender-absent negative control plate (t=0).
  4. Method to detect fluorescence
    1. Plates cultured at 37°C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.
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