Team:Chiba/Calendar-Home/11 October 2008

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[[Team:Chiba/Calendar-Home/10 October 2008|10 October 2008 <]]|[[Team:Chiba/Calendar-Home/12 October 2008|> 12 October 2008]]
[[Team:Chiba/Calendar-Home/10 October 2008|10 October 2008 <]]|[[Team:Chiba/Calendar-Home/12 October 2008|> 12 October 2008]]
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==Laboratory work==
===Team:Demo-Rs===
===Team:Demo-Rs===
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~10 October
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<BR>
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#Receiver(T9002) pre-incubation
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##Receiver:[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908)wascultured in 2mL LB-Amp (37&deg;C,12h)
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##Pre-incubated Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))was plated so as to produce about 1000 colonies.
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#Sender(S03623) pre-incubation
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##Sender:[http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908) was cultured in 50mL  entrifuge tubes in 10mL of LB-Amp (37&deg;C,12h)(2 tubes)
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#Sender Wash
#Sender Wash
##Centrifuged 2 tubes containing([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))at 20&deg;C,3600rpm for 6min and discarded supernatant.
##Centrifuged 2 tubes containing([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))at 20&deg;C,3600rpm for 6min and discarded supernatant.
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##Repeated wash twice.
##Repeated wash twice.
#Creating bacterial plates
#Creating bacterial plates
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##LB-Amp pre-cultured Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908)) tube 1 (10mL) was mixed with LB-Amp-agar(50&deg;C)(10ml)to produce sender containing bacterialplate-1.
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##LB-Amp pre-cultured Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908)) tube(10mL) was mixed with LB-Amp-agar(50&deg;C)(10ml)to produce sender containing bacterialplate.
#Lifted with nitrocellulose
#Lifted with nitrocellulose
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##Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908))containing bacterial plate (1~3) and Sender-absent negative control plate (t=0). Determined the time required for the colonies to fluoresce depending on the bacterial concentration (100 and 1000-fold dilution).
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##Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908))containing bacterial plate and Sender-absent negative control plate (t=0).
#Method to detect fluorescence
#Method to detect fluorescence
##Plates cultured at 37&deg;C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.
##Plates cultured at 37&deg;C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.

Latest revision as of 00:38, 30 October 2008

>Home | Notebook

10 October 2008 <|> 12 October 2008

Laboratory work

Team:Demo-Rs

~10 October

  1. Sender Wash
    1. Centrifuged 2 tubes containing([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))at 20°C,3600rpm for 6min and discarded supernatant.
    2. Added 10mL LB-Amp to each tube.
    3. Repeated wash twice.
  2. Creating bacterial plates
    1. LB-Amp pre-cultured Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908)) tube(10mL) was mixed with LB-Amp-agar(50°C)(10ml)to produce sender containing bacterialplate.
  3. Lifted with nitrocellulose
    1. Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908))containing bacterial plate and Sender-absent negative control plate (t=0).
  4. Method to detect fluorescence
    1. Plates cultured at 37°C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.
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