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Team:Johns Hopkins/Notebook/GROUP 5: MATa Specific Promoters II
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== GROUP 5: MATa Specific Promoters II == | == GROUP 5: MATa Specific Promoters II == | ||
| + | |||
| + | Up to today... | ||
| + | Since then -- we have been troubleshooting and working on getting higher concentrations and using | ||
| + | the correct stock plates. Several delays have occurred,and we should be close to ligating into the | ||
| + | iGEM vector | ||
| + | |||
| + | 8/24 | ||
| + | Restriction Enzyme Digested and the insert appers promising. Ready for sequence submission for Ste 2 promotor. | ||
| + | Status Report by: Alexandra McMillan | ||
| + | |||
| + | 8/21 | ||
| + | Due to low concentrations, we needed to concentrate our DNA before preceeding. | ||
| + | Ethanol precipitation using EtOH/NaAc and 100 ul of miniprep mix was complete of the BBa_K110015 | ||
| + | and BBa_K11009 minipreps. | ||
| + | |||
| + | 8/18 | ||
| + | Miniprep products were < 100 ng/ul. Restriction digest. There appear to be correct | ||
| + | size inserts. Gel image coming. Results look promising. | ||
| + | |||
| + | 8/15 | ||
| + | DNA miniprep of BBa_K110015 and BBa_K11009 cultures | ||
| + | |||
| + | 8/13 | ||
| + | Incubated inoculations of old colony plates | ||
| + | |||
| + | By 7/19/08 | ||
| + | We successfuly transformed both BB 15 and 9 and ran a gel that came out with faint bands. | ||
| + | We digested BB 9 and 15. | ||
Date: 7/11/08 | Date: 7/11/08 | ||
Revision as of 00:59, 30 October 2008
GROUP 5: MATa Specific Promoters II
Up to today... Since then -- we have been troubleshooting and working on getting higher concentrations and using the correct stock plates. Several delays have occurred,and we should be close to ligating into the iGEM vector
8/24 Restriction Enzyme Digested and the insert appers promising. Ready for sequence submission for Ste 2 promotor. Status Report by: Alexandra McMillan
8/21 Due to low concentrations, we needed to concentrate our DNA before preceeding. Ethanol precipitation using EtOH/NaAc and 100 ul of miniprep mix was complete of the BBa_K110015 and BBa_K11009 minipreps.
8/18 Miniprep products were < 100 ng/ul. Restriction digest. There appear to be correct size inserts. Gel image coming. Results look promising.
8/15 DNA miniprep of BBa_K110015 and BBa_K11009 cultures
8/13 Incubated inoculations of old colony plates
By 7/19/08 We successfuly transformed both BB 15 and 9 and ran a gel that came out with faint bands. We digested BB 9 and 15.
Date: 7/11/08 status report by Rick Carrick Part no.: BBa_K110015, Part Description: MFA1 Part Location (in build a genome lab): PCR successful?; Y (on moodle somewhere) Cloning of PCR product successful: Y Sequencing of cloned PCR product successful:N Joining of validated part to adjacent part(s) status: Not done Problems to be solved: None so far Current status of this part: This parts must be restriction enzyme digested and sequenced next.
Date: 7/11/08 status report by Rick Carrick Part no.: BBa_K110009 Part Description: Ste2 Part Location (in build a genome lab): PCR successful?; Y (on moodle somewhere) Cloning of PCR product successful: Y Sequencing of cloned PCR product successful:N Joining of validated part to adjacent part(s) status: Not done Problems to be solved: None so far Current status of this part: This part must be restriction enzyme digested and sequenced next
