Constructs tested

From 2008.igem.org

(Difference between revisions)
 
Line 20: Line 20:
<br>
<br>
'''Experiments to test basal function of XOR gate constructs'''
'''Experiments to test basal function of XOR gate constructs'''
 +
<br>
<br>
<br>
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091173 '''K091173'''] was coplated with the K09100 Lux receiver.  Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091173 '''K091173'''] was coplated with the K09100 Lux receiver.  Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.

Latest revision as of 03:22, 30 October 2008

Home The Team E. nigma Project Parts Submitted to the Registry Notebook



Experiment to test the function of old and new Lac promoters


Each of the promoters is used to drive expression of GFP so that fluorescence can be used to measure promoter function. The data demonstrate that the new Lac promoters have improved function over the existing wild type promoter in the Registry.

Trial1.png </center>

Experiments to test basal function of XOR gate constructs

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091173 K091173] was coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.

9100 K82.JPG


XOR gate construct [http://partsregistry.org/wiki/index.php?title=Part:BBa_K091182 K091182] coplated with the K09100 Lux receiver. Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.

9100 K73.JPG