"
User:University of Washington/1 July 2008
From 2008.igem.org
(New page: == == Team:University_of_Washington/Notebook#Notebook) |
|||
| (5 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | == | + | == LuxR from AraC and TetR == |
| + | |||
| + | - AraC sequences matched the biobrick (BBa_R0080). | ||
| + | |||
| + | == LuxR from pLAc == | ||
| + | |||
| + | - Miniprep done on R0010 and J04430 transformed e.coli. DNA was submitted for sequencing of the parts. | ||
| + | |||
| + | - Part I0462 was again transformed into a different e.coli strain. | ||
| + | |||
| + | |||
| + | == BioBrick Promoter Measurements == | ||
| + | |||
| + | - All overnight cultures showed successful transformation by growing on plates with kanamycin. | ||
| + | |||
| + | - Single colonies were added to 5 mL of TSY media, then incubated at 37 degrees Celsius in a rotator for 5 hours. | ||
| + | |||
| + | - 750 uL of the incubated cells were added to 750 uL of 40% glycerol, incubated at room temperature for 30 minutes, then stored at -80 degrees Celsius. | ||
| + | |||
| + | - The remaining cultures were placed back into the 37 degree incubator to grow overnight. | ||
| + | |||
| + | == Conjugation == | ||
| + | |||
| + | ·Transform RP4 into S17-1 cells | ||
| + | |||
| + | |||
| + | ---- | ||
[[Team:University_of_Washington/Notebook#Notebook]] | [[Team:University_of_Washington/Notebook#Notebook]] | ||
Latest revision as of 23:03, 2 July 2008
Contents |
LuxR from AraC and TetR
- AraC sequences matched the biobrick (BBa_R0080).
LuxR from pLAc
- Miniprep done on R0010 and J04430 transformed e.coli. DNA was submitted for sequencing of the parts.
- Part I0462 was again transformed into a different e.coli strain.
BioBrick Promoter Measurements
- All overnight cultures showed successful transformation by growing on plates with kanamycin.
- Single colonies were added to 5 mL of TSY media, then incubated at 37 degrees Celsius in a rotator for 5 hours.
- 750 uL of the incubated cells were added to 750 uL of 40% glycerol, incubated at room temperature for 30 minutes, then stored at -80 degrees Celsius.
- The remaining cultures were placed back into the 37 degree incubator to grow overnight.
Conjugation
·Transform RP4 into S17-1 cells
Team:University_of_Washington/Notebook#Notebook
