Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)

From 2008.igem.org

Latest revision as of 09:53, 30 October 2008

Results and Discussion

Reaction temparature:37°C,09/12

Sender culture：1000μL,Receiver culture:1000μL

Fig.1　　
12/09.E.coli strain,senders:JW1908,BBa_T9002:JW1908,Reaction temparature:37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Sender culture：100μL,Receiver culture：1000μL

Fig.2　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

• Fig. 1,Fig. 2
• Results
  • Green fluorescence intensity didn't increase in the culture containing BBa_K0840010(plac+CinI+LVA).We thought BBa_K084010 didn't work properly or LuxR gene didn't interact with signal molecules synthesized by BBa_K084010.
  • Others responded at the same time(4 hours after induction).Final fluorescence intensity differd.

Reaction temparature:37°C

Sender culture：500μLm,Receiver culture：500μL

Fig.3　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Fig.4　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

• Fig. 3
• Results
  • Response time and final fluorescence intensity showed no significant difference.
• Discussion
  • We thought AHL concentration was quickly reached the threshold concentration.The fluorescence intensity increased as gfp maturured.

• Fig. 4
• Results
  • No significant difference in fluorescence intensity between the culture containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.
• Discussion
  • We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Reaction temparature:30°C

Sender culture：500μL,Receiver culture：500μL

Fig.5　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Fig.6　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

• Fig. 6
• Results
  • No significant difference in fluorescence intensity between the cultures containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.
• Discussion
  • We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Sender culture：100μL,Receiver culture：1000μL

Fig.7　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Fig.8　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

• Fig.7
• Results
  • No significant difference in fluorescence intensity between the culture containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.
• Discussion
  • We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Sender culture：10μL,Receiver culture：1000μL

Fig.9　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

• Fig.9
• Results
  • The final fluorescence intensity of the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] transformed cells which express LVA-tagged RhlI protein was lower than the culture containing cells which express untagged RhlI protein.It shows that LVA-tagged RhlI protein was degraded by LVA-specific protase.However,fluorescence intensity of both cultures increased at the same time.
• Discussion
  • We thought it was because the AHL concentration was quickly reached the threshold concentration and cells began to express gfp.After 2 hours,fluorescence intensity started to increase.

>Back to Sender experiment and result

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers